Faculty of Medicine, Department of Pediatric Surgery, Harran University, Sanliurfa, Turkey.
J Pediatr Surg. 2013 Apr;48(4):716-23. doi: 10.1016/j.jpedsurg.2012.08.015.
The aim of this study was to investigate the effectiveness of hyaluronic acid on the prevention of esophageal damage and stricture formation after experimental caustic (alkaline) esophageal injury in rats.
Twenty-one Wistar albino rats were randomly divided into three groups. A caustic esophageal burn was created following the Gehanno model: Group l (n=7) underwent operation, but no injury; Group 2 (n=7) was injured and left untreated; and Group 3 (n=7) was injured and treated with hyaluronic acid, first topically and then orally by gavage (2×0.3mL; 12.5mg/mL for 7days). The caustic esophageal burn was created by instilling 25% NaOH into the distal esophagus. All rats were euthanized on day 22 for evaluation. The efficacy of hyaluronic acid treatment was assessed histopathologically and biochemically via blood determination of the total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), and sulfhydryl group (SH) and lipid hydroperoxidase (LOOH) levels. Statistical analyses were performed.
Weight gain was significantly lower in Group 2 than in the other two groups (P<0.05). The mean stenosis index, histopathologic damage score, TAS, TOS, OSI, and SH and LOOH levels were higher in Group 2 than in the other two groups. The mean stenosis index, inflammation, TAS, SH and OSI in Group 2 were significantly different than those in the other two groups (P<0.05).
Hyaluronic acid treatment is effective in treating damage and preventing strictures after caustic esophageal burn in rats.
本研究旨在探讨透明质酸在预防大鼠实验性腐蚀性（碱性）食管损伤后食管损伤和狭窄形成中的作用。
将 21 只 Wistar 白化大鼠随机分为三组。采用 Gehanno 模型造成腐蚀性食管烧伤：第 1 组（n=7）仅行手术，但不造成损伤；第 2 组（n=7）造成损伤但不治疗；第 3 组（n=7）造成损伤后用透明质酸治疗，先局部后经口灌胃（2×0.3mL；12.5mg/mL 共 7 天）。通过向远端食管内注入 25%NaOH 造成腐蚀性食管烧伤。所有大鼠于第 22 天处死进行评估。通过测定总抗氧化状态（TAS）、总氧化状态（TOS）、氧化应激指数（OSI）、巯基（SH）和脂质过氧化物（LOOH）水平的血液生化指标，评估透明质酸治疗的效果。进行统计学分析。
第 2 组大鼠体重增加明显低于其他两组（P<0.05）。第 2 组狭窄指数、组织病理学损伤评分、TAS、TOS、OSI 以及 SH 和 LOOH 水平均高于其他两组。第 2 组狭窄指数、炎症、TAS、SH 和 OSI 与其他两组相比差异均有统计学意义（P<0.05）。
透明质酸治疗对大鼠腐蚀性食管烧伤后损伤的治疗和狭窄的预防有效。
