Takishita Y, Hiraiwa K, Nagayama M
First Department of Oral and Maxillofacial Surgery, School of Dentistry, Tokushima University.
J Biochem. 1990 Apr;107(4):592-6. doi: 10.1093/oxfordjournals.jbchem.a123091.
We obtained terminally differentiated chondrocytes in monolayer culture from chick embryonal growth plates, and examined the effect of retinoic acid on these cells. The cells treated with retinoic acid ceased type X collagen synthesis and showed decreased calcium incorporation into cell layers. Retinoic acid tended to stimulate proliferation of the cultured chondrocytes. It also increased DNA accumulation dose-dependently in the range from 1 nM to 1 microM. DNA synthesis in the growth phase and confluency was stimulated within 10 h after addition of 0.1 microM retinoic acid. [3H]Retinoic acid binding, which was inhibited by simultaneous addition of excess unlabeled retinoic acid, was detected in both the cytosolic and nuclear fractions of the chondrocytes. The retinoic acid binding capacity of the nuclear fraction was increased by pretreating the cells with retinoic acid. These results indicate that retinoic acid binds to both the cytosolic and nuclear fractions of cultured chondrocytes, and induces their proliferation and dedifferentiation.
我们从鸡胚生长板的单层培养物中获得了终末分化的软骨细胞，并研究了视黄酸对这些细胞的影响。用视黄酸处理的细胞停止了X型胶原蛋白的合成，并显示出细胞层中钙掺入量减少。视黄酸倾向于刺激培养的软骨细胞增殖。在1 nM至1 microM范围内，它还剂量依赖性地增加了DNA积累。添加0.1 microM视黄酸后10小时内，生长阶段和汇合状态下的DNA合成受到刺激。在软骨细胞的胞质和核部分均检测到[3H]视黄酸结合，同时添加过量未标记视黄酸可抑制该结合。用视黄酸预处理细胞可增加核部分的视黄酸结合能力。这些结果表明，视黄酸与培养的软骨细胞的胞质和核部分均结合，并诱导其增殖和去分化。
