Department of Hepatobiliary Surgery, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy, China.
Cell Biol Int. 2013 May;37(5):436-42. doi: 10.1002/cbin.10057. Epub 2013 Feb 18.
The spindle, composed of microtubules and associated proteins, serves as major machinery for cell division. Aurora B is a chromosome passenger protein that has important functions in mitosis. Its dynamic distribution at mitosis goes along with spindle structure and dynamics. We used the siRNA technique to knockdown protein expression and immunofluorescence technique to follow Aurora B during mitosis. Aurora B regulates microtubule plus ends as mitosis progresses, including both kinetochore and polar microtubules. Interactions between Aurora B and polar microtubule plus ends lead to failure in cytokinesis and abnormal midbody structure. We think Aurora B may not only play a role as kinase, but regulate microtubule plus ends.
纺锤体由微管和相关蛋白组成，是细胞分裂的主要机制。Aurora B 是一种染色体乘客蛋白，在有丝分裂中具有重要功能。其在有丝分裂中的动态分布与纺锤体结构和动力学相伴随。我们使用 siRNA 技术敲低蛋白表达，并通过免疫荧光技术在有丝分裂过程中跟踪 Aurora B。Aurora B 调节微管的正极，包括着丝粒微管和极性微管，随着有丝分裂的进行。Aurora B 与极性微管正极之间的相互作用导致胞质分裂失败和异常的中间体结构。我们认为 Aurora B 可能不仅作为激酶发挥作用，而且调节微管的正极。
