飞行时间二次离子质谱（TOF-SIMS）在血管生物学中的应用。

Use of TOF-SIMS in vascular biology.

作者信息

Mas Sebastián, Pérez Raúl, Egido Jesús

机构信息

Vascular Research Lab IIS-Fundación Jiménez Díaz, Instituto de Investigacion Sanitaria Fundacion Jimenez Diaz (IIS-FJD), Universidad Autónoma de Madrid, Madrid, Spain.

出版信息

Methods Mol Biol. 2013;1000:33-43. doi: 10.1007/978-1-62703-405-0_3.

DOI:10.1007/978-1-62703-405-0_3

PMID:23585082

Abstract

Secondary ion mass spectrometry is a technique used for surface analysis. The recent introduction of metal cluster ionization sources opened the possibility to probe biological tissues, due to its reduced in-source fragmentation. Despite its limitations in mass range of the molecules analyzed, a combination of minimal sample processing, sensibility, and unsurpassed lateral resolution makes TOF-SIMS a good tool for molecular imaging in pathology.

摘要

二次离子质谱分析法是一种用于表面分析的技术。由于其源内碎片化程度降低，金属簇电离源的近期引入为探测生物组织开辟了可能性。尽管其在分析分子的质量范围方面存在局限性，但最小化样品处理、灵敏度和无与伦比的横向分辨率相结合，使得飞行时间二次离子质谱分析法成为病理学中分子成像的良好工具。