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调控双斑蟋(Gryllus bimaculatus)消化道中淀粉酶、纤维素酶和几丁质酶的分泌。

Regulation of amylase, cellulase and chitinase secretion in the digestive tract of the two-spotted field cricket, Gryllus bimaculatus.

机构信息

Department of Animal Ecology I, University of Bayreuth, Germany.

出版信息

Arch Insect Biochem Physiol. 2013 Jun;83(2):69-85. doi: 10.1002/arch.21092. Epub 2013 Apr 12.

DOI:10.1002/arch.21092
PMID:23585293
Abstract

The secretion of amylase and cellulase in Gryllus bimaculatus is determined by increased food intake, whereby shortly after molting food consumption increases. About half of the standing amylase concentration (activity) in the endothelial cells can be secreted within 30 min. The peak of amylase and cellulase secretion that occurs in the photophase is related to the feeding peak in the previous scotophase. The secretion of chitinase on the other hand is primarily controlled by the molting cycle. Only amylase secretion was affected by calcium in the incubation medium, suggesting an apocrine release mechanism. Refeeding experiments (after 5 days without food) suggest that the release of amylase in response to a nutrient in the lumen (glucose) is not due to simple stimulation of exocytosis, but rather a stimulation of synthesis.

摘要

东亚飞蝗的淀粉酶和纤维素酶的分泌受食物摄入量增加的影响,即在蜕皮后不久,食物的消耗量增加。内皮细胞中约一半的基础淀粉酶浓度(活性)可以在 30 分钟内被分泌出来。在光期发生的淀粉酶和纤维素酶分泌峰值与前一个暗期的摄食峰值有关。另一方面,几丁质酶的分泌主要受蜕皮周期的控制。只有淀粉酶的分泌受到孵育介质中钙的影响,提示可能存在顶浆分泌机制。再投喂实验(在 5 天没有食物的情况下)表明,对腔(葡萄糖)中营养物质的反应性淀粉酶的释放不是由于胞吐作用的简单刺激,而是合成的刺激。

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