Ye Le, Du Fei-Yan, Wang Xue-Hui, Wang Yu
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences , Guangzhou, Guangdong , China and.
Mitochondrial DNA. 2014 Apr;25(2):87-8. doi: 10.3109/19401736.2013.784749. Epub 2013 Apr 16.
The complete mitochondrial genome of the black-dotted grouper Epinephelus stictus was first determined in this study. It is 16,524 bp in length, including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and one control region, with the typical gene arrangement in vertebrates. There are some variations in the initiate and terminal codons among 13 protein-coding genes. The 12S rRNA and 16S rRNA genes are 952 and 1705 bp, respectively. The tRNA-Ser2 gene lacked DHC arm and could not fold into a typical clover-leaf secondary structure. The base composition is different between each portion of mitochondrial genome.
本研究首次测定了黑点石斑鱼（Epinephelus stictus）的完整线粒体基因组。其长度为16,524 bp，包括13个蛋白质编码基因、两个核糖体RNA基因、22个转运RNA基因和一个控制区，具有脊椎动物典型的基因排列。13个蛋白质编码基因的起始密码子和终止密码子存在一些差异。12S rRNA和16S rRNA基因分别为952 bp和1705 bp。tRNA-Ser2基因缺少二氢尿嘧啶臂，无法折叠成典型的三叶草二级结构。线粒体基因组各部分的碱基组成不同。
