Department of Medical Biophysics, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, Canada.
Dent Mater. 2013 Jun;29(6):645-55. doi: 10.1016/j.dental.2013.03.013. Epub 2013 Apr 12.
To synthesize and characterize machinable, bioactive glass-ceramics (GCs) suitable for dental implant applications.
A glass in the SiO2-Al2O3-CaO-CaF2-K2O-B2O3-La2O3 system was synthesized by wet chemical methods, followed by calcination, melting and quenching. Crystallization kinetics were determined by differential thermal analysis (DTA). GC discs were produced by cold pressing of the glass powder and sintered using schedules determined by DTA. The crystalline phases and microstructure of GC samples were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), respectively. Dynamic Young's modulus (E), true hardness (Ho), fracture toughness (KIC) and brittleness index (BI) were evaluated. Bioactivity was studied by examining the formation of hydroxyapatite (HA) on the GC surfaces after soaking in simulated body fluid (SBF). Attachment and proliferation of MC3T3-E1 osteoblastic cells were assessed in vitro.
Miserite [KCa5(Si2O7)(Si6O15)(OH)F] was the main crystalline phase of the GC with additional secondary phases. Microstructural studies revealed interlocking lath-like crystalline morphology. E, Ho, and KIC values for the GCs were 96±3 GPa, 5.27±0.26 GPa and 4.77±0.27 MPa m(0.5), respectively. The BI was found to be 1.11±0.05 μm(-0.5), indicating outstanding machinability. An HA surface layer was formed on the GC surfaces when soaked in SBF, indicating potential bioactivity. MC3T3-E1 cells exhibited attachment, spreading and proliferation on GC surfaces, demonstrating excellent biocompatibility.
We present a novel approach for the synthesis of miserite GC with the physical and biological properties required for non-metallic dental implant applications.
合成并表征适用于牙科植入物应用的可加工生物活性玻璃陶瓷（GC）。
通过湿化学方法合成 SiO2-Al2O3-CaO-CaF2-K2O-B2O3-La2O3 系统中的玻璃，然后进行煅烧、熔融和淬火。通过差示热分析（DTA）确定结晶动力学。通过冷压玻璃粉末生产 GC 圆盘，并使用 DTA 确定的时间表进行烧结。通过 X 射线衍射（XRD）和扫描电子显微镜（SEM）分别对 GC 样品的晶体相和微观结构进行了表征。评估了动态杨氏模量（E）、真实硬度（Ho）、断裂韧性（KIC）和脆性指数（BI）。通过在模拟体液（SBF）中浸泡后检查 GC 表面形成的羟基磷灰石（HA）来研究生物活性。体外评估 MC3T3-E1 成骨细胞的附着和增殖。
Miserite [KCa5(Si2O7)(Si6O15)(OH)F] 是 GC 的主要晶相，还有其他次要相。微观结构研究表明存在互锁的薄片状晶体形态。GC 的 E、Ho 和 KIC 值分别为 96±3 GPa、5.27±0.26 GPa 和 4.77±0.27 MPa m(0.5)。BI 为 1.11±0.05 μm(-0.5)，表明可加工性优异。当在 SBF 中浸泡时，GC 表面形成了一层 HA 表面层，表明具有潜在的生物活性。MC3T3-E1 细胞在 GC 表面附着、伸展和增殖，表现出优异的生物相容性。
我们提出了一种合成 miserite GC 的新方法，其具有用于非金属牙科植入物应用的物理和生物学特性。
