College of Materials Science and Engineering, Sichuan University, Chengdu 610064, People's Republic of China.
J Biomed Mater Res B Appl Biomater. 2010 Apr;93(1):194-202. doi: 10.1002/jbm.b.31574.
Three new chemical compositions based on the CaO-MgO-SiO(2) system were designed first, and then three novel glass-ceramics (M1, M2, and M3) were prepared by sol-gel method. X-ray diffraction analysis confirmed that they were predominantly composed of akermanite, wollastonite, and dicalcium silicate crystalline phases. The coefficient of thermal expansion (CTE) of M2 was 10.79 x 10(-6) degrees C(-1), closest to that of Ti-6Al-4V alloy, and the Young's modulus of M2 was 29.73 GPa, similar to that of the cortical bone. The bioactivity in vitro of M2 was evaluated by investigating its bonelike hydroxyapatite (HA)-formation ability in simulated body fluid (SBF), and the biocompatibility in vitro was detected by osteoblast proliferation, differentiation, and adhesion assay. The results revealed that M2 possessed bonelike carbonated hydroxyapatite (CHA)-formation ability in SBF and could significantly stimulate cell proliferation and differentiation. Furthermore, osteoblasts adhered and spread well on M2, indicating good bioactivity and biocompatibility in vitro.
首先设计了基于 CaO-MgO-SiO(2) 系统的三种新型化学成分，然后通过溶胶-凝胶法制备了三种新型玻璃陶瓷（M1、M2 和 M3）。X 射线衍射分析证实，它们主要由硅灰石、硅灰石和二硅酸钙晶相组成。M2 的热膨胀系数（CTE）为 10.79 x 10(-6) 摄氏度(-1)，与 Ti-6Al-4V 合金最接近，M2 的杨氏模量为 29.73 GPa，与皮质骨相似。通过研究其在模拟体液（SBF）中的类骨羟基磷灰石（HA）形成能力，评估了 M2 的体外生物活性，并通过成骨细胞增殖、分化和黏附试验检测了其体外生物相容性。结果表明，M2 在 SBF 中具有类骨碳酸羟基磷灰石（CHA）形成能力，并能显著刺激细胞增殖和分化。此外，成骨细胞在 M2 上黏附和铺展良好，表明其具有良好的体外生物活性和生物相容性。
