胶原酶预防术后腹腔内粘连的效果。

Effectiveness of collagenase in preventing postoperative intra-abdominal adhesions.

机构信息

Necmettin Erbakan University, Meram Medical Faculty, Department of General Surgery, Konya 42080, Turkey.

出版信息

Int J Surg. 2013;11(6):487-91. doi: 10.1016/j.ijsu.2013.04.001. Epub 2013 Apr 12.

DOI:10.1016/j.ijsu.2013.04.001

PMID:23587652

Abstract

INTRODUCTION

The purpose of this study is to investigate the effectiveness of Collagenase clastridiopeptidase an enzyme preparation used in enzymatic debridement in preventing adhesions brought about by peritoneal damage.

METHODS

The study covers a total of 40 rats in 4 groups each having 10 rats. Group 1: The control group. Group 2: Normal saline group. Group 3: Sterile Novuxol group. Group 4: The group where the intraperitoneal and systemic effects of sterile Novuxol were investigated. Adhesion frequency and grades were scored on the post-op 11th day according to Granat. Blood work including hemoglobin, aspartate aminotransferase, alanine aminotransferase, urea, creatinine, and albumin level measurements were performed. Toxicity was investigated histopathologically through samples taken from the liver and the peritoneum from Group 4.

RESULTS

Adhesion frequency was found to be 80% on the right and 90% on the left for Group 1, while it was 50% on both left and right for Group 2, and 30% on the right and 10% on the left for Group 3. Adhesion stages were found to be 1: 2.35 ± 1.42 for Group 1, 0.31 ± 1.15 for Group 2, and 0.20 ± 0.41 for Group 3. Adhesion stage of the Sterile Novuxol Group was lower than all the other groups (p < 0.05). Biochemical and hematological parameters were similar in all groups (p > 0.05). Histopathological analysis revealed no hepatotoxicity.

CONCLUSIONS

According to the results of our study, we believe that Sterile Novuxol can be a good anti-adhesive agent considering its ease of use, non-toxicity, and effectiveness.

摘要

简介

本研究旨在探讨胶原酶梭菌蛋白酶制剂（一种用于酶清创的酶制剂）在预防腹膜损伤引起的粘连方面的有效性。

方法

本研究共纳入 40 只大鼠，分为 4 组，每组 10 只。第 1 组：对照组。第 2 组：生理盐水组。第 3 组：无菌 Novuxol 组。第 4 组：研究无菌 Novuxol 的腹腔内和全身作用的组。根据 Granat 的标准，在术后第 11 天对粘连频率和等级进行评分。进行血红蛋白、天门冬氨酸氨基转移酶、丙氨酸氨基转移酶、尿素、肌酐和白蛋白水平的血液检查。通过对第 4 组的肝脏和腹膜进行组织病理学检查来研究毒性。

结果

第 1 组右侧粘连频率为 80%，左侧为 90%，第 2 组双侧均为 50%，第 3 组右侧为 30%，左侧为 10%。第 1 组粘连等级为 1:2.35±1.42，第 2 组为 0.31±1.15，第 3 组为 0.20±0.41。无菌 Novuxol 组的粘连等级低于其他所有组（p<0.05）。所有组的生化和血液学参数均相似（p>0.05）。组织病理学分析未发现肝毒性。