Department of Materials Engineering, School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Colloids Surf B Biointerfaces. 2013 Aug 1;108:345-51. doi: 10.1016/j.colsurfb.2013.02.022. Epub 2013 Feb 24.
A precise spatial multicellular polymer hydrogel matrix was prepared by successive assembly of cell-laden hydrogel layers alternated by hydrogel layers without cells based on the spontaneous hydrogel formation between 2 aqueous polymer solutions. The polymers used were a water-soluble 2-methacryloyloxyethyl phosphorylcholine polymer bearing phenylboronic acid groups (PMBV) and poly(vinyl alcohol) (PVA). Each cell-laden layer was deposited as a cell-laden PMBV solution on a PMBV/PVA precursor film. PMBV/PVA multilayer hydrogel was stacked on the top of a cell-laden layer by sequential coating with spinning of the PMBV and PVA solutions. This process allowed the formation of the PMBV/PVA multilayer hydrogel with finely controlled thickness. Finally, we succeeded in cell patterning by using a multilayer hydrogel matrix, forming a sandwich of 2 cell-laden layers separated by a PMBV/PVA multilayer hydrogel. The cells remained alive during the spinning process and maintained their metabolism for at least 24h. This precise spatial multicellular PMBV/PVA hydrogel can be used to examine interactions between many different cells and construct customized microenvironments for multicellular co-cultures.
通过将含有细胞的水凝胶层与不含细胞的水凝胶层交替组装，基于两种水相聚合物溶液之间的自发水凝胶形成，制备了精确空间多细胞聚合物水凝胶基质。所用的聚合物是一种带有苯硼酸基团的水溶性 2-甲基丙烯酰氧乙基磷酸胆碱聚合物（PMBV）和聚乙烯醇（PVA）。每个含细胞层都是作为含细胞的 PMBV 溶液沉积在 PMBV/PVA 前体膜上的。通过顺序旋涂 PMBV 和 PVA 溶液，在含细胞层的顶部堆叠 PMBV/PVA 多层水凝胶。该过程允许形成具有精细控制厚度的 PMBV/PVA 多层水凝胶。最后，我们通过使用多层水凝胶基质成功地进行了细胞图案化，形成了由 2 个含细胞层夹在 PMBV/PVA 多层水凝胶之间的三明治结构。在旋涂过程中，细胞保持存活并维持其代谢至少 24 小时。这种精确空间多细胞 PMBV/PVA 水凝胶可用于研究许多不同细胞之间的相互作用，并构建用于多细胞共培养的定制微环境。
