Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2013 Sep 15;47:271-7. doi: 10.1016/j.bios.2013.03.039. Epub 2013 Mar 27.
A simple approach based on exfoliating and disintegrating treatments for graphite oxide, followed by hydrothermal synthesis, was developed to prepare water-soluble graphene quantum dots (GQDs). The as-prepared GQDs exhibited bright blue emission under ultraviolet irradiation (∼365nm), and showed an excitation-independent photoluminescence feature. More importantly, a newly anodic electrochemiluminescence (ECL) was observed from the water-soluble GQDs with H2O2 as coreactant for the first time, and the ECL induced a strong light emission at a low potential (ca. 0.4V vs. Ag/AgCl). The ECL mechanism is investigated in detail. Employing SiO2 nanospheres as signal carrier, a novel SiO2/GQDs ECL signal amplification labels were synthesized based on which a ultrasensitive ECL aptamer sensor was proposed. Under the optimized experimental conditions, the proposed ECL aptamer sensor exhibited excellent analytical performance for adenosine triphosphate (ATP) determination, ranging from 5.0×10(-12) to 5.0×10(-9)molL(-1) with the detection limit of 1.5×10(-12)molL(-1). Due to the low cytotoxicity and excellent biocompatibility, GQDs are demonstrated to be an eco-friendly material as well as excellent ECL labeling agents for biosensor.
一种基于剥离和分解处理氧化石墨，然后进行水热合成的简单方法，被开发用来制备水溶性石墨烯量子点（GQDs）。所制备的 GQDs 在紫外光（约 365nm）照射下呈现出明亮的蓝色发射，并表现出激发独立的光致发光特性。更重要的是，首次观察到水溶性 GQDs 在 H2O2 作为共反应物时具有新的阳极电化学发光（ECL），并且 ECL 在低电位（约 0.4V 相对于 Ag/AgCl）下诱导强发光。详细研究了 ECL 机制。利用 SiO2 纳米球作为信号载体，合成了一种新型的 SiO2/GQDs ECL 信号放大标记物，并在此基础上提出了一种超灵敏的 ECL 适体传感器。在优化的实验条件下，所提出的 ECL 适体传感器对三磷酸腺苷（ATP）的测定表现出优异的分析性能，检测范围为 5.0×10(-12)至 5.0×10(-9)molL(-1)，检测限为 1.5×10(-12)molL(-1)。由于其低细胞毒性和优异的生物相容性，GQDs 被证明是一种环保材料，也是生物传感器的优异 ECL 标记物。
