State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Org Lett. 2013 May 3;15(9):2132-5. doi: 10.1021/ol400619t. Epub 2013 Apr 16.
A choline labeled pyrene probe (Py-Ch) was designed and synthesized. Poly(vinylsulfonate) (PVS) could induce Py-Ch aggregation. The aggregation and deaggregation process could be finely controlled by the acetylcholinesterase (AChE) enzymatic hydrolysis of Py-Ch. The resulting excimer-monomer transition provided a facile way for real-time AChE activity fluorometric assay and inhibitor screening.
设计并合成了一种标记有胆碱的芘探针（Py-Ch）。聚（乙烯磺酸盐）（PVS）可以诱导 Py-Ch 聚集。通过 Py-Ch 的乙酰胆碱酯酶（AChE）酶解，可以精细控制聚集和解聚集过程。所得的激基缔合物-单体转变为实时 AChE 活性荧光测定和抑制剂筛选提供了一种简便的方法。
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