优化甲硫醇生物传感器的过氧化氢检测。

Optimization of hydrogen peroxide detection for a methyl mercaptan biosensor.

机构信息

State Key Laboratory of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

出版信息

Sensors (Basel). 2013 Apr 15;13(4):5028-39. doi: 10.3390/s130405028.

Several kinds of modified carbon screen printed electrodes (CSPEs) for amperometric detection of hydrogen peroxide (H₂O₂) are presented in order to propose a methyl mercaptan (MM) biosensor. Unmodified, carbon nanotubes (CNTs), cobalt phthalocyanine (CoPC), Prussian blue (PB), and Os-wired HRP modified CSPE sensors were fabricated and tested to detect H₂O₂, applying a potential of +0.6 V, +0.6 V, +0.4 V, -0.2 V and -0.1 V (versus Ag/AgCl), respectively. The limits of detection of these electrodes for H₂O₂ were 3.1 μM, 1.3 μM, 71 nM, 1.3 μM, 13.7 nM, respectively. The results demonstrated that the Os-wired HRP modified CSPEs gives the lowest limit of detection (LOD) for H₂O₂ at a working potential as low as -0.1 V. Os-wired HRP is the optimum choice for establishment of a MM biosensor and gives a detection limit of 0.5 μM.

为了提出一种甲硫醇（MM）生物传感器，介绍了几种用于电流检测过氧化氢（H₂O₂）的改性碳丝网印刷电极（CSPE）。制备了未改性、碳纳米管（CNT）、钴酞菁（CoPC）、普鲁士蓝（PB）和 Os 布线 HRP 修饰的 CSPE 传感器，并分别施加+0.6 V、+0.6 V、+0.4 V、-0.2 V 和-0.1 V（相对于 Ag/AgCl）的电势以检测 H₂O₂。这些电极对 H₂O₂的检测限分别为 3.1 μM、1.3 μM、71 nM、1.3 μM、13.7 nM。结果表明，Os 布线 HRP 修饰的 CSPE 在工作电势低至-0.1 V 时给出了对 H₂O₂的最低检测限（LOD）。Os 布线 HRP 是建立 MM 生物传感器的最佳选择，检测限为 0.5 μM。