Department of Surgical Gastroenterology, Hvidovre Hospital, University of Copenhagen, Hvidovre, Denmark.
Int J Biol Markers. 2013 Jun 28;28(2):226-30. doi: 10.5301/jbm.5000017.
At present plasma tissue inhibitor of metalloproteinases-1 (TIMP-1) is undergoing validation as a biological marker in colorectal cancer (CRC). The clinical implementation of plasma TIMP-1 in prognosis, prediction, screening and monitoring CRC requires robust information as to the influence of preanalytical factors, including inter- and intrapersonal biological variations. The aim of the present study was to evaluate the possible effects of smoking on the level of TIMP-1 in plasma from healthy subjects.
Forty-six never-smokers and 48 daily smokers participated in the 13-week study. Smokers were randomized into 3 groups of 16 subjects each: one group continued to smoke, a second group refrained from smoking and used a transdermal nicotine patch, and a third group refrained from smoking and used placebo patches. Plasma TIMP-1 levels were determined using ELISA.
No significant differences in TIMP-1 levels could be shown between the smoking group, the 2 different abstinent smoking groups and the never-smoking group.
Smoking does not appear to have any influence on TIMP-1 levels in plasma collected from healthy subjects.
目前，血浆组织金属蛋白酶抑制剂-1（TIMP-1）正在作为结直肠癌（CRC）的生物标志物进行验证。血浆 TIMP-1 在 CRC 的预后、预测、筛查和监测中的临床应用需要有强有力的信息来证明分析前因素的影响，包括个体内和个体间的生物学差异。本研究的目的是评估吸烟对健康受试者血浆中 TIMP-1 水平的可能影响。
46 名从不吸烟者和 48 名每日吸烟者参加了为期 13 周的研究。吸烟者被随机分为 3 组，每组 16 人：一组继续吸烟，另一组戒烟并使用透皮尼古丁贴片，第三组戒烟并使用安慰剂贴片。使用 ELISA 法测定血浆 TIMP-1 水平。
吸烟组、2 个不同的戒烟组和从不吸烟者组之间的 TIMP-1 水平无显著差异。
吸烟似乎对健康受试者血浆中收集的 TIMP-1 水平没有影响。
