Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Sagamihara, 252-5201, Japan.
Folia Microbiol (Praha). 2013 Nov;58(6):607-13. doi: 10.1007/s12223-013-0250-x. Epub 2013 Apr 18.
Although the absence of intervening sequences (IVSs) within the 23S rRNA genes in Campylobacter lari isolates has been described, there are apparently no reports regarding correlations between the nucleotide sequences of 23S rRNA genes and erythromycin (Ery) susceptibility in C. lari isolates. Here, we determined the minimum inhibitory concentrations of 35 C. lari isolates [n = 19 for urease-positive thermophilic Campylobacter (UPTC); n = 16 urease-negative (UN) C. lari] obtained from Asia, Europe, and North America. We found that the 18 isolates were resistant to the Ery (defined as ≧8 μg/mL), and three isolates, UPTC A1, UPTC 92251, and UPTC 504, showed increased resistance (16 μg/mL). No correlations between the IVSs in the helix 45 region within the 23S rRNA gene sequences and Ery resistance were identified in the C. lari isolates examined. In addition, no point mutations occurred at any expected or putative position within the V domain in the isolates. In conclusion, antibiotic resistance against the macrolide erythromycin is mediated through an alternative pathway to that described above.
虽然已经描述了弯曲杆菌属拉里菌分离株的 23S rRNA 基因中不存在间隔序列（IVSs），但显然没有关于弯曲杆菌属拉里菌分离株的 23S rRNA 基因核苷酸序列与红霉素（Ery）敏感性之间相关性的报道。在这里，我们确定了 35 株弯曲杆菌属拉里菌分离株的最小抑菌浓度[19 株为脲酶阳性嗜热弯曲杆菌（UPTC）；16 株为脲酶阴性（UN）弯曲杆菌属拉里菌]，这些分离株分别来自亚洲、欧洲和北美。我们发现，18 株对 Ery 有耐药性（定义为≧8μg/mL），3 株，即 UPTC A1、UPTC 92251 和 UPTC 504，表现出了更高的耐药性（16μg/mL）。在我们研究的弯曲杆菌属拉里菌分离株中，未发现 23S rRNA 基因序列中螺旋 45 区内 IVSs 与 Ery 耐药性之间存在相关性。此外，在分离株的 V 结构域内，没有发生任何预期或可能的位置的点突变。总之，对大环内酯类红霉素的抗生素耐药性是通过上述途径以外的途径介导的。
