脓毒症患者与非脓毒症性全身炎症反应综合征患者血浆miR-146a的差异表达。
Differential expression of plasma miR-146a in sepsis patients compared with non-sepsis-SIRS patients.
作者信息
Wang Lina, Wang Hua-Cheng, Chen Cha, Zeng Jianming, Wang Qian, Zheng Lei, Yu Huan-DU
机构信息
Department of Laboratory Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006; ; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515;
出版信息
Exp Ther Med. 2013 Apr;5(4):1101-1104. doi: 10.3892/etm.2013.937. Epub 2013 Jan 30.
Sepsis is a subtype of systemic inflammatory response syndrome (SIRS), which is characterized by infection. Circulating microRNAs (miRNAs), including miR-150, miR-146a and miR-223, are potential biomarkers of sepsis. In this study, we demonstrated that measuring the relative expression of miR-146a/U6 in plasma, using the 2 method, provides a method for differentiating between sepsis and non-sepsis-SIRS. We observed a significant increase in miR-146a expression in the initial cohort of 6 non-sepsis-SIRS patients compared to the 4 sepsis patients (P=0.01) and in the second cohort of 8 non-sepsis-SIRS patients compared to the 10 sepsis patients (P=0.027). Additionally, we identified that sodium citrate and ethylenediaminetetraacetic acid (EDTA) K may be used as anticoagulant reagents. Generation of a standard curve is not necessary in these diagnostic tests, unless the standard of normalization is carefully selected. Thus we provide more detailed guidance for the clinical use of circulating miRNA biomarkers.
脓毒症是全身性炎症反应综合征（SIRS）的一种亚型，其特征为感染。循环微RNA（miRNA），包括miR-150、miR-146a和miR-223，是脓毒症的潜在生物标志物。在本研究中，我们证明，使用2−ΔΔCt法测量血浆中miR-146a/U6的相对表达，可为区分脓毒症和非脓毒症SIRS提供一种方法。我们观察到，在初始队列中，6例非脓毒症SIRS患者的miR-146a表达相较于4例脓毒症患者显著升高（P = 0.01）；在第二个队列中，8例非脓毒症SIRS患者的miR-146a表达相较于10例脓毒症患者显著升高（P = 0.027）。此外，我们确定柠檬酸钠和乙二胺四乙酸（EDTA）钾可用作抗凝剂。在这些诊断测试中，无需生成标准曲线，除非仔细选择标准化标准。因此，我们为循环miRNA生物标志物的临床应用提供了更详细的指导。
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