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实验性免疫受损犬鼻拭子中犬流感H3N2病毒的长期脱落

Prolonged shedding of the canine influenza H3N2 virus in nasal swabs of experimentally immunocompromised dogs.

作者信息

Hong Minki, Kang Bokyu, Na Woonsung, An Dongjun, Moon Hyoungjoon, Kim Doo-Jin, Oh Jinsik, Park Seong-Jun, Poo Haryoung, Kim Jeong-Ki, Kim Jongman, Song Daesub

机构信息

Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea. ; University of Science and Technology, Daejeon, Korea.

出版信息

Clin Exp Vaccine Res. 2013 Jan;2(1):66-8. doi: 10.7774/cevr.2013.2.1.66. Epub 2013 Jan 15.

Abstract

PURPOSE

The avian origin canine influenza virus H3N2 has been recently isolated and found to be currently in dog population in South Korea and China. The purpose of this study was to clarify the relationship between immunosuppressive glucocorticoids used in veterinary clinical practice and viral shedding pattern of influenza in dogs.

MATERIALS AND METHODS

Eight conventional beagle dogs were divided into control infection group and immunocompromised group. Dogs of both groups were infected with H3N2 canine influenza virus (2×10(6.0) EID50/0.1 mL). Dogs in immunocompromised group were given orally 3.0 mg/kg prednisolone for 7 days. Virus shedding was monitored using real-time polymerase chain reaction. After necropsy, histopathologic lesions were compared.

RESULTS

We found that immunocompromised dogs exhibited more prolonged (8 days vs. 13 days) and higher magnitude viral shedding than control group (peak titer of viral shedding 4.6 vs. 5.5 EID50).

CONCLUSION

Restricted use of immunosuppressive drugs in the clinical setting might help control the rapid spread of H3N2 through local dog populations.

摘要

目的

源自禽类的犬流感病毒H3N2最近已被分离出来,且发现目前在韩国和中国的犬类群体中存在。本研究的目的是阐明兽医临床实践中使用的免疫抑制性糖皮质激素与犬流感病毒 shedding 模式之间的关系。

材料与方法

将8只常规比格犬分为对照感染组和免疫受损组。两组犬均感染H3N2犬流感病毒(2×10(6.0) EID50/0.1 mL)。免疫受损组的犬口服3.0 mg/kg泼尼松龙,持续7天。使用实时聚合酶链反应监测病毒 shedding。尸检后,比较组织病理学病变。

结果

我们发现,免疫受损的犬比对照组表现出更长时间(8天对13天)和更高水平的病毒 shedding(病毒 shedding 的峰值滴度为4.6对5.5 EID50)。

结论

在临床环境中限制使用免疫抑制药物可能有助于控制H3N2在当地犬类群体中的快速传播。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ab3/3623504/ecd2b9aea80b/cevr-2-66-g001.jpg

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