Liu Guihua, Qin Wei, Liu Honghe, Zhu Zhou
Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
Wei Sheng Yan Jiu. 2013 Jan;42(1):61-6.
To develop a quick and accurate method for simultaneous determining the multi-residues of hormones in foods of animal origin by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and to investigate these residues in swine, bovine, egg and milk collected from local markets in Shenzhen.
The sample was firstly extracted with acetonitrile, and then subjected to solid-phase extraction clean-up using HLB-NH2 cartridges after defatted with hexane, lastly detected by high performance liquid chromatography-tandem mass spectrometry. Identification was achieved by electrospray ionization (ESI) in both positive and negative mode using multiple reaction monitoring. Quantification was performed by internal standard calibration.
The study showed that there was a certain amount of endogenous hormones in collected samples. The limits of quantification were 0.5 - 1.0 microg/kg for 20 hormones in swine, bovine, egg and milk. Average recoveries were 60.4% -118.2%, and the relative standard deviations were 2.5% - 16.2%.
This method was quick and accurate which could be used for determination of hormones in foods of animal origin (such as swine, bovine, egg and milk).
建立一种快速、准确的液相色谱-串联质谱法（LC-MS/MS）同时测定动物源性食品中多种激素残留的方法，并对深圳当地市场采集的猪、牛、蛋、奶中的这些残留进行调查。
样品首先用乙腈提取，然后用正己烷脱脂后，使用HLB-NH2柱进行固相萃取净化，最后用高效液相色谱-串联质谱法检测。采用电喷雾电离（ESI）在正、负离子模式下通过多反应监测进行鉴定。通过内标校准进行定量。
研究表明，所采集的样品中存在一定量的内源性激素。猪、牛、蛋、奶中20种激素的定量限为0.5 - 1.0微克/千克。平均回收率为60.4% - 118.2%，相对标准偏差为2.5% - 16.2%。
该方法快速、准确，可用于测定动物源性食品（如猪、牛、蛋、奶）中的激素。
