[Effects of angelica keiskei chalcone on expression of glucose transporter proteins in liver and skeletal muscle cells of type 2 diabetic rats].

OBJECTIVE

To investigate the effect of angelica keiskei chalcone (AC) on the expression of glucose transporter2 (Glut2) in liver and glucose transporter 4 (Glut4) in skeletal muscle cells of rats with type2 diabetes.

METHODS

Made type2 diabetic rat models and divided into high, middle, low dose groups and diabetic control group randomly which were given chalcone 30, 10, 5 and 0 mg/kg BW respectively. Four groups were fed with the diet containing high fat. After four weeks, fasting blood glucose was determined by glucose oxidase method. Insulin was assessed by radio-immunity method. The expression levels of Glut2 in liver and Glut4 in skeletal muscle cells were analyzed by immunohistochemistry method.

RESULTS

After analyzed by Image-Pro Plus on immunohistochemistry pictures, the expression levels of Glut2 in liver and Glut4 in skeletal muscle cells in high group were (0.036 +/- 0.0030) and (0.063 +/- 0.0139), which were significantly higher than the model control group (P < 0.05). The levels of fasting blood glucose and insulin in high dose group were (12.3 +/- 1.64) mmol/L and (25.65 +/- 3.34) microIU/ml, significantly lower than those of the model control group (P < 0.05).

CONCLUSION

Angelica keiskei chalcone may increase the expression levels of Glut2 in liver and Glut4 in skeletal muscle cells, decrease fasting blood glucose and insulin of type 2 diabetic rats and improve their insulin resistance condition.