[液相色谱法和气相色谱法同时测定食品中的三种抗氧化剂丁基羟基茴香醚、二丁基羟基甲苯和特丁基对苯二酚]

[Simultaneous determination of three antioxidants BHA, BHT and TBHQ in food by liquid chromatography and gas chromatography].

作者信息

Yang Jie, Fang Congrong, Yang Dajin

机构信息

China National Centre for Food Safety Risk Assessment, Beijing 100021, China.

出版信息

Wei Sheng Yan Jiu. 2013 Jan;42(1):114-8.

PMID:23596721

Abstract

OBJECTIVE

To develop a method for determining 3 kinds of antioxidants in food in same time with HPLC and GC.

METHODS

Firstly, extracted fat with petroleum ether, then extracted antioxidants with 13 ml methnol, centrifuged, repeated the above procedure twice, combined the extracts, next evaporated to 5 ml, constanted volume to 10 ml, then kepted in freezer (- 1h), lastly, the supernatant injected into high performance liquid chromatography (HPLC) and Gas chromatography (GC) which had been optimized condition.

RESULTS

The limits of quantitation for BHA,BHT and TBHQ were 0.002, 0.010 and 0.002g/kg respectively in HPLC, The limits of quantitation for BHA, BHT and TBHQ were 0.003, 0.002 and 0.005 g/kg respectively in GC, The mean recoveries at the two spiked levels were 82.8% - 109.0%. There were no significant difference between HPLC and GC.

CONCLUSION

The method can be used to determine the antioxidants in food accurately and sensitively, the comparative results are constant between liquid and gas chromatography.

摘要

目的

建立一种同时采用高效液相色谱法（HPLC）和气相色谱法（GC）测定食品中3种抗氧化剂的方法。

方法

首先用石油醚提取脂肪，然后用13ml甲醇提取抗氧化剂，离心，重复上述步骤两次，合并提取物，接着蒸发至5ml，定容至10ml，然后置于冰箱中（-1h），最后将上清液注入已优化条件的高效液相色谱仪（HPLC）和气相色谱仪（GC）中。

结果

在HPLC中，丁基羟基茴香醚（BHA）、二丁基羟基甲苯（BHT）和特丁基对苯二酚（TBHQ）的定量限分别为0.002、0.010和0.002g/kg；在GC中，BHA、BHT和TBHQ的定量限分别为0.003、0.002和0.005g/kg，两个加标水平的平均回收率为82.8% - 109.0%。HPLC和GC之间无显著差异。