College of Civil Engineering, Nanjing Forestry University, Nanjing 210037, China.
Bioresour Technol. 2013 Jun;137:349-57. doi: 10.1016/j.biortech.2013.03.137. Epub 2013 Mar 27.
To find an appropriate method for sulfate-rich wastewater containing ethanol and acetate with COD/sulfate ratio of 1, a UASB reactor was operated for more than 180 days. The influences of HRT (hydraulic retention time) and OLR (organic loading rate) on organics and sulfate removal, gas production, and electrons utilization were investigated. The sludge activity and microorganism composition were also determined. The results indicated that this system removed more than 80% of COD and 30% of sulfate with HRT above 6h and OLR below 12.3 gCOD/L d. Further HRT decrease caused volatile fatty acids accumulation and performance deterioration. Except at HRT of 2h, COD and electron flow were mostly utilized by methane-producing archaea (MPA), and methane yield remained in the range of 0.18-0.24 LCH4/gCOD. Methane was mainly generated by Methanosaeta concilii GP6 with acetate as substrate, whereas sulfate was mainly reduced by incomplete-oxidizing Desulfovibrio species with ethanol as substrate.
为了找到一种合适的方法来处理含有乙醇和乙酸且 COD/硫酸盐比为 1 的富硫酸盐废水，使用 UASB 反应器进行了 180 多天的运行。考察了水力停留时间（HRT）和有机负荷率（OLR）对有机物和硫酸盐去除、产气量和电子利用的影响，并确定了污泥活性和微生物组成。结果表明，当 HRT 大于 6h 且 OLR 小于 12.3 gCOD/L·d 时，该系统可去除超过 80%的 COD 和 30%的硫酸盐。进一步降低 HRT 会导致挥发性脂肪酸积累和性能恶化。除 HRT 为 2h 外，COD 和电子流主要被产甲烷古菌（MPA）利用，甲烷产率保持在 0.18-0.24 LCH4/gCOD 范围内。甲烷主要由乙酸作为底物的 Methanosaeta concilii GP6 生成，而硫酸盐主要由以乙醇作为底物的不完全氧化脱硫菌还原。
