Tohoku University, Graduate School of Environmental Studies, Aoba 6-6-06, Aramaki, Aoba-ku, Sendai, Miyagi, Japan.
Tohoku University, Department of Civil and Environmental Engineering, Aoba 6-6-06, Aobayama, Aoba-ku, Sendai, Miyagi, Japan.
Chemosphere. 2015 Dec;140:184-90. doi: 10.1016/j.chemosphere.2014.08.028. Epub 2014 Sep 16.
The influence of Cephalexin (CLX), Tetracycline (TC), Erythromycin (ERY) and Sulfathiazole (ST) on methane-producing archaea (MPA) and sulfate-reducing bacteria (SRB) in anaerobic sludge was investigated using acetate or ethanol as substrate. With antimicrobial concentrations below 400mgL(-1), the relative specific methanogenic activity (SMA) was above 50%, so that the antimicrobials exerted slight effects on archaea. However ERY and ST at 400mgL(-1) caused a 74.5% and 57.6% inhibition to specific sulfidogenic activity (SSA) when the sludge granules were disrupted and ethanol used as substrate. After disruption, microbial tolerance to antimicrobials decreased, but the rate at which MPA utilized acetate and ethanol increased from 0.95gCOD·(gVSS⋅d)(-1) to 1.45gCOD·(gVSS⋅d)(-1) and 0.90gCOD·(gVSS⋅d)(-1) to 1.15gCOD·(gVSS⋅d)(-1) respectively. The ethanol utilization rate for SRB also increased after disruption from 0.35gCOD·(gVSS⋅d)(-1) to 0.46gCOD·(gVSS⋅d)(-1). Removal rates for CLX approaching 20.0% and 25.0% were obtained used acetate and ethanol respectively. The disintegration of granules improved the CLX removal rate to 65% and 78%, but ST was not removed during this process.
采用乙酸或乙醇作为底物，研究了头孢氨苄（CLX）、四环素（TC）、红霉素（ERY）和磺胺噻唑（ST）对厌氧污泥中产甲烷古菌（MPA）和硫酸盐还原菌（SRB）的影响。在抗菌剂浓度低于 400mgL(-1)时，相对特定产甲烷活性（SMA）高于 50%，因此抗菌剂对古菌的影响很小。然而，ERY 和 ST 在 400mgL(-1)时，当污泥颗粒被破坏且使用乙醇作为底物时，对特定的硫化物生成活性（SSA）分别抑制了 74.5%和 57.6%。破坏后，微生物对抗菌剂的耐受性降低，但 MPA 利用乙酸和乙醇的速率从 0.95gCOD·(gVSS⋅d)(-1)增加到 1.45gCOD·(gVSS⋅d)(-1)和 0.90gCOD·(gVSS⋅d)(-1)增加到 1.15gCOD·(gVSS⋅d)(-1)。破坏后，SRB 对乙醇的利用率也从 0.35gCOD·(gVSS⋅d)(-1)增加到 0.46gCOD·(gVSS⋅d)(-1)。分别用乙酸和乙醇，CLX 的去除率接近 20.0%和 25.0%。颗粒的崩解将 CLX 的去除率提高到 65%和 78%，但在此过程中 ST 未被去除。
