National Institute of Science and Technology-INCT for Bioanalytics, Institute of Chemistry, University of Campinas-Unicamp, P.O. Box 6154, 13083-970, Campinas, SP, Brazil.
Talanta. 2013 Jan 30;104:187-92. doi: 10.1016/j.talanta.2012.11.009. Epub 2012 Nov 16.
The present speciation method reports the determination of inorganic arsenic forms, using metallic furnace hydride generation atomic absorption spectrometry. The inorganic As speciation is carried out using mild conditions for hydride formation, such as slightly acid pH media (4.50) and low tetrahydridoborate(1-) concentration (0.1% (w/v)). Limits of detection and quantification of 2.0 and 6.6 μg L(-1) of iAs(III) are obtained using optimized conditions. Additionally, microwave-assisted extraction using water as solvent is carried out to provide the appropriate environment for As species extraction as well as impeding inter-conversion between species. With these analytical strategies, As was accurately determined (at 99.9% confidence level) in water and plankton samples.
本形态分析方法采用金属炉氢化物发生原子吸收光谱法报告无机砷形态的测定。采用温和的氢化物形成条件进行无机砷形态分析，例如稍酸性 pH 介质（4.50）和低四硼氢化物（1-）浓度（0.1%（w/v））。在优化条件下，可获得 iAs(III) 的检测限和定量限分别为 2.0 和 6.6 μg L(-1)。此外，还使用水作为溶剂进行微波辅助萃取，以提供适当的环境用于提取砷形态，同时阻碍形态之间的相互转化。通过这些分析策略，可以准确地测定水和浮游生物样品中的砷（置信水平为 99.9%）。
