Kuang Yan, Cai Jing, Li Donglin, Han Qin, Cao Jin, Wang Zehua
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022; ; Department of Obstetrics and Gynecology, First Affiliated Hospital, GuangXi Medical University, Nanning 530021, P.R. China.
Oncol Lett. 2013 Apr;5(4):1149-1154. doi: 10.3892/ol.2013.1158. Epub 2013 Jan 28.
Dicer is a key enzyme that processes microRNA (miRNA) precursors into their mature form, enabling them to regulate gene expression. However, the effects of Dicer on the biological behavior of cancer cells remain largely unclear. In this study, it was demonstrated that Dicer down-regulation promoted cell proliferation, migration and cell cycle progression in A2780 and SKOV3 ovarian cancer cells. Furthermore, Dicer expression was significantly decreased in cisplatin-resistant A2780 cells (A2780/DDP) compared with parental A2780 cells. Knockdown of Dicer by RNA interference decreased the sensitivity of A2780 cells to cisplatin. Moreover, EZH2 depletion by short hairpin RNA (shRNA) increased the expression of Dicer . Our data suggest that Dicer is involved in numerous biological/pathological processes, including drug resistance in ovarian cancer, and that its expression may be regulated by EZH2.
Dicer是一种关键酶，可将微小RNA（miRNA）前体加工成成熟形式，使其能够调节基因表达。然而，Dicer对癌细胞生物学行为的影响仍 largely不清楚。在本研究中，证明了Dicer下调促进了A2780和SKOV3卵巢癌细胞的增殖、迁移和细胞周期进程。此外，与亲本A2780细胞相比，顺铂耐药的A2780细胞（A2780/DDP）中Dicer表达显著降低。通过RNA干扰敲低Dicer降低了A2780细胞对顺铂的敏感性。此外，短发夹RNA（shRNA）介导的EZH2缺失增加了Dicer的表达。我们的数据表明，Dicer参与了包括卵巢癌耐药性在内的众多生物学/病理过程，并且其表达可能受EZH2调控。
