Wang Li-Qiang, Shi Hua-Shan, Wang Yong-Sheng
Department of Cell Biology, College of Life Science, Sichuan University, Chengdu 610064, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 Jan;44(1):46-8, 75.
To prepare water soluble curcumin liposome and investigate its anti-tumour and antiangiogenic effects.
Liposomal curcumin was prepared by alcohol injection method. Proliferation inhibition to murin Lewis lung cancer cell line LL/2 of curcumin liposome was evaluated by MTT assay. Apoptosis and cell cycle arrest induced by liposomal curcumin were analysed by flow cytometry. Anti-tumour effects were investigated in a murine lung cancer model, and the anti-angiogenic effect was determined by aginate encapsulation assay.
In vitro, liposomal curcumin inhibits the proliferation of LL/2 cells and induces apoptosis and cell cycle arrest. In vivo, the systemic administration of liposomal curcumin resulted in the inhibition of tumour. Aginate encapsulation assay revealed angiogenesis was decreased by curcumin liposome.
The curcumin liposome treatment can significantly inhibit tumour growth in vivo.
制备水溶性姜黄素脂质体并研究其抗肿瘤和抗血管生成作用。
采用乙醇注入法制备姜黄素脂质体。通过MTT法评估姜黄素脂质体对小鼠Lewis肺癌细胞系LL/2的增殖抑制作用。采用流式细胞术分析姜黄素脂质体诱导的细胞凋亡和细胞周期阻滞。在小鼠肺癌模型中研究其抗肿瘤作用，并通过海藻酸盐包囊试验测定抗血管生成作用。
在体外，姜黄素脂质体抑制LL/2细胞的增殖并诱导细胞凋亡和细胞周期阻滞。在体内，全身给予姜黄素脂质体导致肿瘤受到抑制。海藻酸盐包囊试验显示姜黄素脂质体可减少血管生成。
姜黄素脂质体治疗可显著抑制体内肿瘤生长。
