Semënov D Iu, Boriskova M E, Zaraĭskiĭ M I, Saburova I Iu, Pankova P A, Farafonova U V, Bykov M A
Vopr Onkol. 2012;58(5):649-52.
A possibility of detection of BRAF mutation has been studied by means of polymerase chain reaction in punctate from the thyroid node with the aim of diagnostics of high-differentiated cancer on pre-operative stage. A study includes 94 patients with nodal lesions of the thyroid. According to cytological investigation of material, received by fine-needle aspiration biopsy, papillary thyroid cancer was revealed in 47 patients, follicular tumor-in 35 and nodal colloid goiter in 12 patients. In case of papillary thyroid cancer data of genetic analysis showed the following indices of sensitivity, specificity and diagnostic accuracy: 86%, 100% and 92% correspondingly. Detection of BRAF mutation could be used in pre-operative differential diagnostics of high-differentiated cancer and benign nodal lesions of the thyroid.
为了在术前阶段诊断高分化癌，已通过聚合酶链反应研究了甲状腺结节穿刺物中BRAF突变的检测可能性。该研究纳入了94例甲状腺结节性病变患者。根据细针穿刺活检获取材料的细胞学检查，47例患者诊断为甲状腺乳头状癌，35例为滤泡性肿瘤，12例为结节性胶样甲状腺肿。对于甲状腺乳头状癌，遗传分析数据显示敏感性、特异性和诊断准确性指标分别为86%、100%和92%。BRAF突变检测可用于甲状腺高分化癌与良性结节性病变的术前鉴别诊断。
