针对大肠杆菌外膜蛋白A的小鼠单克隆抗体。
Mouse monoclonal antibody specific for outer membrane protein A of Escherichia coli.
作者信息
Abe Yoshihiro, Haruta Ikuko, Yanagisawa Naoko, Yagi Junji
机构信息
Department of Microbiology and Immunology, Tokyo Women's Medical University, Tokyo, Japan.
出版信息
Monoclon Antib Immunodiagn Immunother. 2013 Feb;32(1):32-5. doi: 10.1089/mab.2012.0069.
Abstract
Outer membrane protein A (OmpA) is a major outer membrane protein of Escherichia coli and other Enterobacteriaeae. Although the structural features of OmpA have been well studied, its roles in the pathogenesis of various bacterial infections have not been fully elucidated. Here, we report the generation of mouse monoclonal antibody (MAb) 49.4-15, which specifically recognizes OmpA of E. coli, using immunoblot and confocal microscopic examinations. MAb 49.4-15 might be a useful tool for studying the expression and function of E. coli OmpA.
摘要
外膜蛋白A（OmpA）是大肠杆菌和其他肠杆菌科细菌的一种主要外膜蛋白。尽管OmpA的结构特征已得到充分研究，但其在各种细菌感染发病机制中的作用尚未完全阐明。在此，我们报告了小鼠单克隆抗体（MAb）49.4 - 15的产生，通过免疫印迹和共聚焦显微镜检查，该抗体可特异性识别大肠杆菌的OmpA。MAb 49.4 - 15可能是研究大肠杆菌OmpA表达和功能的有用工具。
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本文引用的文献
1
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FEBS J. 2012 Mar;279(6):894-909. doi: 10.1111/j.1742-4658.2012.08484.x. Epub 2012 Feb 10.
2
Outer membrane protein A and OprF: versatile roles in Gram-negative bacterial infections.外膜蛋白 A 和 OprF：在革兰氏阴性菌感染中的多种作用。
FEBS J. 2012 Mar;279(6):919-31. doi: 10.1111/j.1742-4658.2012.08482.x. Epub 2012 Feb 10.
3
Mechanism of anchoring of OmpA protein to the cell wall peptidoglycan of the gram-negative bacterial outer membrane.OmpA 蛋白与革兰氏阴性菌外膜细胞壁肽聚糖的锚定机制。
FASEB J. 2012 Jan;26(1):219-28. doi: 10.1096/fj.11-188425. Epub 2011 Sep 30.
4
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Biophys Chem. 2011 Nov;159(1):194-204. doi: 10.1016/j.bpc.2011.06.013. Epub 2011 Jul 6.
5
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6
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7
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Lab Invest. 2010 Apr;90(4):577-88. doi: 10.1038/labinvest.2010.40. Epub 2010 Feb 8.
9
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Appl Environ Microbiol. 2005 Dec;71(12):8008-15. doi: 10.1128/AEM.71.12.8008-8015.2005.
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