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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochim Biophys Acta. 1975 Apr 7;385(2):412-20. doi: 10.1016/0304-4165(75)90370-0.

The nature of the acid-soluble mannosamine intermediates was investigated in bovine thyroid gland slices after incubation with a tracer amount of [14C]mannosamine plus 10 mM carrier mannosamine. 2. These radioactive intermediates were separated by ion exchange, columns, characterized by paper chromatography and were identified as N-acetylmannosamine-6-P. The radioactivity associated with these acid-soluble compounds constituted about 17 percent of the added [14C]mannosamine. 3. [14C]Mannosamine was incorporated as sialic acid only into thyroglobulin.

在用微量[14C]甘露糖胺加10 mM载体甘露糖胺孵育牛甲状腺切片后，研究了酸溶性甘露糖胺中间体的性质。2. 这些放射性中间体通过离子交换柱分离，用纸色谱法进行表征，并被鉴定为N-乙酰甘露糖胺-6-P。与这些酸溶性化合物相关的放射性约占添加的[14C]甘露糖胺的17%。3. [14C]甘露糖胺仅作为唾液酸掺入甲状腺球蛋白中。

Biochim Biophys Acta. 1975 Apr 7;385(2):412-20. doi: 10.1016/0304-4165(75)90370-0.

The nature of the acid-soluble mannosamine intermediates was investigated in bovine thyroid gland slices after incubation with a tracer amount of [14C]mannosamine plus 10 mM carrier mannosamine. 2. These radioactive intermediates were separated by ion exchange, columns, characterized by paper chromatography and were identified as N-acetylmannosamine-6-P. The radioactivity associated with these acid-soluble compounds constituted about 17 percent of the added [14C]mannosamine. 3. [14C]Mannosamine was incorporated as sialic acid only into thyroglobulin.

在用微量[14C]甘露糖胺加10 mM载体甘露糖胺孵育牛甲状腺切片后，研究了酸溶性甘露糖胺中间体的性质。2. 这些放射性中间体通过离子交换柱分离，用纸色谱法进行表征，并被鉴定为N-乙酰甘露糖胺-6-P。与这些酸溶性化合物相关的放射性约占添加的[14C]甘露糖胺的17%。3. [14C]甘露糖胺仅作为唾液酸掺入甲状腺球蛋白中。