Féray C, Zignego A L, Samuel D, Bismuth A, Reynes M, Tiollais P, Bismuth H, Brechot C
U 163-Hybridotest, Institut Pasteur, Paris, France.
Transplantation. 1990 Jun;49(6):1155-8. doi: 10.1097/00007890-199006000-00025.
We have investigated the recurrence of Hepatitis B virus (HBV) in 30 patients treated by orthotopic liver transplantation and given high doses of anti-HBs immunoglobulin. The polymerase chain reaction (PCR) assay showed no evidence of HBV DNA sequences in the liver of 23/24 patients who remained serum HBsAg-negative during a mean follow-up of 13 months (2-24 months) after OLT. However, the liver scored positive in all the 6 individuals in whom HBsAG reappeared. The PCR assay identified HBV DNA sequences in the peripheral blood mononuclear cells of 7 of 11 subjects who were serum HBsAG-negative and liver HBV DNA-negative by PCR. Therefore, this application of the sensitive PCR assay demonstrates persistent infection of PBMC in the absence of liver HBV--thus OLT provides a model for studying the interaction between HBV, PBMC, and the liver.
我们对30例接受原位肝移植并给予大剂量抗-HBs免疫球蛋白治疗的患者的乙型肝炎病毒（HBV）复发情况进行了研究。聚合酶链反应（PCR）检测显示，在原位肝移植（OLT）后平均13个月（2 - 24个月）的随访期间，24例血清HBsAg持续阴性的患者中，有23例肝脏未检测到HBV DNA序列。然而，在所有6例HBsAg再次出现的个体中，肝脏检测呈阳性。PCR检测在11例血清HBsAg阴性且肝脏PCR检测HBV DNA阴性的受试者中的7例外周血单个核细胞中鉴定出HBV DNA序列。因此，这种敏感PCR检测方法的应用表明，在肝脏无HBV感染的情况下，外周血单个核细胞存在持续感染——因此，OLT为研究HBV、外周血单个核细胞和肝脏之间的相互作用提供了一个模型。
