College of Pharmacy, Chonnam National University, Gwangju 500-757, South Korea.
FEBS Lett. 2013 Jun 5;587(11):1610-6. doi: 10.1016/j.febslet.2013.04.009. Epub 2013 Apr 18.
Sec14 family homologs are the major yeast phosphatidylinositol/phosphatidylcholine transfer proteins regulating lipid metabolism and vesicle trafficking. The structure of Saccharomyces cerevisiae Sfh3 displays a conserved Sec14 scaffold and reveals determinants for the specific recognition of phosphatidylinositol ligand. Apo-Sfh3 forms a dimer through the hydrophobic interaction of gating helices. Binding of phosphatidylinositol leads to dissociation of the dimer into monomers in a reversible manner. This study suggests that the substrate induced dimer-monomer transformation is an essential part of lipid transfer cycles by Sfh3.
Sec14 家族同源物是调节脂质代谢和囊泡运输的主要酵母磷脂酰肌醇/磷脂酰胆碱转移蛋白。酿酒酵母 Sfh3 的结构显示出保守的 Sec14 支架，并揭示了特异性识别磷脂酰肌醇配体的决定因素。脱辅基 Sfh3 通过门控螺旋的疏水相互作用形成二聚体。磷脂酰肌醇的结合以可逆的方式导致二聚体解离成单体。本研究表明，底物诱导的二聚体-单体转化是 Sfh3 进行脂质转移循环的重要组成部分。
