Rushton A R, Dawson G
Biochim Biophys Acta. 1975 Apr 18;388(1):92-105.
Evidence is presented for the existence of three distinct mammalian glycosphingolipid beta-galactosidase responsible for the hydrolysis of galactosylceramide, lactosylceramide and GM1 gangliside, respectively. Activity toward the (L-3-H)galactose-labeled substrates differed with respect to pH optimum, thermostability, effect of NaCl and inhibition by glycosides and related glycosphinglpids. Comparison of these enzymic acitivites in cultured mouse cell line LMTK- and human beta-galactosiddases could probably be detected in future experiments with somatic cell hybrids (formed by the fusion of these two cell strains by specifically inhibiting activity of mouse origin.
有证据表明存在三种不同的哺乳动物糖鞘脂β-半乳糖苷酶，分别负责水解半乳糖神经酰胺、乳糖神经酰胺和GM1神经节苷脂。对（L-3-H）半乳糖标记底物的活性在最适pH、热稳定性、NaCl的影响以及糖苷和相关糖鞘脂的抑制作用方面存在差异。在培养的小鼠细胞系LMTK-和人β-半乳糖苷酶中比较这些酶活性，在未来用体细胞杂种（通过这两种细胞株融合形成，通过特异性抑制小鼠来源的活性）进行的实验中可能检测到。
