AVT-Biochemical Engineering, RWTH Aachen University, Worringer Weg 1, Aachen, 52074, Germany,
Adv Biochem Eng Biotechnol. 2014;138:45-60. doi: 10.1007/10_2013_188.
: Orbitally shaken single-use reactors are promising reactors for upstream processing, because they fulfill three general requirements for single-use equipment. First, the design of the disposable parts is inherently simple and cost-efficient, because no complex built-in elements such as baffles or rotating stirrers are required. Second, the liquid distribution induced by orbital shaking is well-defined and accurately predictable. Third, the scale-up from small-scale systems, where shaken bioreactors are commonly applied, is simple and has been successfully proven up to the cubic meter scale. However, orbitally shaken single-use reactors are only suitable for certain applications such as cultivating animal or plant cells with low oxygen demand. Thus, detailed knowledge about the performance of such systems on different scales is essential to exploit their full potential. This article presents an overview about opportunities and limitations of shaken single-use reactors.
轨道摇床式一次性反应器是一种有前途的上游处理用反应器，因为它们满足了一次性设备的三个一般要求。首先，一次性部件的设计本质上简单且具有成本效益，因为不需要复杂的内置元件，如挡板或旋转搅拌器。其次，轨道摇床引起的液体分布是明确的，并且可以准确预测。第三，从小型系统（通常应用摇床式生物反应器的地方）扩展规模很简单，并且已经成功证明可以扩展到立方米规模。然而，轨道摇床式一次性反应器仅适用于某些应用，例如培养低需氧量的动植物细胞。因此，详细了解此类系统在不同规模下的性能对于充分发挥其潜力至关重要。本文概述了摇床式一次性反应器的机遇和限制。
