Hubbard K, Ozer H L
Age (Omaha). 1999 Apr;22(2):65-9. doi: 10.1007/s11357-999-0008-1.
Model systems implementing various approaches to immortalize cells have led toward further understanding of replicative senescence and carcinogenesis. Human diploid cells have a limited life span, termed replicative senescence. Because cells are terminally growth arrested during replicative senescence, it has been suggested that it acts as a tumor suppression mechanism as tumor cells exhibit an indefinite life span and are immortal. The generation of immortal cells lines, by the introduction of SV40 and human papillomavirus (HPV) sequences into cells, has provided invaluable tools to dissect the mechanisms of immortalization. We have developed matched sets of nonimmortal and immortal SV40 cell lines which have been useful in the identification of novel growth suppressor genes (SEN6) as well as providing a model system for the study of processes such as cellular aging, apoptosis, and telomere stabilization. Thus, their continued use is anticipated to lead to insights into other processes, which are effected by the altered expression of oncogenes and growth suppressors.
实施各种使细胞永生化方法的模型系统有助于进一步理解复制性衰老和致癌作用。人类二倍体细胞具有有限的寿命，称为复制性衰老。由于细胞在复制性衰老期间最终会停止生长，因此有人提出这是一种肿瘤抑制机制，因为肿瘤细胞具有无限寿命且是永生的。通过将SV40和人乳头瘤病毒（HPV）序列引入细胞来产生永生细胞系，为剖析永生化机制提供了宝贵的工具。我们已经开发了匹配的非永生和永生SV40细胞系，这些细胞系有助于鉴定新的生长抑制基因（SEN6），并为研究细胞衰老、凋亡和端粒稳定等过程提供了一个模型系统。因此，预计继续使用它们将有助于深入了解受癌基因和生长抑制因子表达改变影响的其他过程。
