Institute of Veterinary Medicine, University of Cluj Napoca, St Calea, Manastur 3, RO-3400, Cluj-Napoca.
Mycotoxin Res. 2005 Mar;21(1):65-7. doi: 10.1007/BF02954821.
Ruminal zearalenone (ZEA) degradation in "in vitro" digestion was examined in different variants using the Hohenheimer Gas Test. First, the mycotoxin degradation was measured using squeezed rumen fluid from solid digesta of the dorsal sack and free rumen fluid from the ventral sack, respectively. Then free rumen, fluid of the ventral sac was used with addition of concentrate and mixtures of concentrate with sun-flower oil and starch, respectively. Within 24 hours ZEA was degraded down to 63% and 49% of the initial concentration when incubated with solid and fluid rumen digesta, respectively. Using additives and rumen fluid concentrate for incubation, concentrate, concentrate with oil and concentrate with starch, respectively, a reduction of ZEA to 46, 56 and 37% of the initial toxin concentration was observed.
采用 Hohenheimer 气体测试法,研究了不同条件下瘤胃中玉米赤霉烯酮(ZEA)的“体外”消化降解情况。首先,分别使用来自背部囊固体消化物的挤出瘤胃液和来自腹囊的游离瘤胃液来测量真菌毒素的降解情况。然后,分别使用添加精料和精料与葵花籽油及淀粉混合物的腹囊游离瘤胃液进行试验。24 小时内,与固体和液体瘤胃消化物分别孵育时,ZEA 的初始浓度分别降低了 63%和 49%。当使用添加剂和瘤胃液浓缩物进行孵育时,与对照相比,浓缩物、含油浓缩物和含淀粉浓缩物中 ZEA 分别减少到初始毒素浓度的 46%、56%和 37%。