提取纯化对高效液相色谱分析中桔霉素回收率的影响。

Influence of extract purification on the recovery of citrinin in the HPLC analysis.

机构信息

IGV Institut für Getreideverarbeitung GmbH, Arthur-Scheunert-Allee 40-41, D-14558, Bergholz-Rehbrücke.

出版信息

Mycotoxin Res. 2001 Jun;17 Suppl 2:165-9. doi: 10.1007/BF03036428.

Abstract

Various analytical methods of citrinin determination are known from publications which cannot be applied without any difficulties. Low fluctuating recovery rates of standard additions, an insufficient extract purification for rye, and a rather low sensitivity based on improper HPLC conditions are being observed if the published methods are applied. An easily applicable, reliable and sensitive HPLC method in the lower ppb range on the basis of a solid phase extraction combined with a HPLC gradient which allows for a sensitive fluorescence detection, is under development. The results already achieved are described in the following.

摘要

各种曲酸分析方法已在出版物中报道，但这些方法并非没有任何困难就能应用。如果应用已发表的方法，则观察到标准添加回收率波动低、黑麦提取净化不足以及基于不适当的 HPLC 条件灵敏度较低。正在开发一种基于固相萃取和 HPLC 梯度相结合的、可实现灵敏荧光检测的、适用于较低 ppb 范围的简单、可靠和灵敏的 HPLC 方法。以下将描述已经取得的结果。