Laboratório de Erros Inatos do Metabolismo, Programa de Pós-graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
Oxid Med Cell Longev. 2013;2013:607610. doi: 10.1155/2013/607610. Epub 2013 Mar 27.
trans-Glutaconic acid (tGA) is an unsaturated C5-dicarboxylic acid which may be found accumulated in glutaric aciduria type I, whose pathophysiology is still uncertain. In the present work it was investigated the in vitro effect of increasing tGA concentrations on neurochemical and oxidative stress parameters in rat cerebral cortex. We observed that Na(+), K(+)-ATPase activity was reduced by tGA, but not creatine kinase, respiratory chain complex IV, and ATP synthase activities. On the other hand, tGA significantly increased lipid peroxidation (thiobarbituric acid-reactive species levels and spontaneous chemiluminescence), as well as protein oxidative damage (oxidation of sulfhydryl groups). tGA also significantly decreased nonenzymatic antioxidant defenses (TRAP and reduced glutathione levels). Our data suggest that tGA may be neurotoxic in rat brain.
反式-谷氨酸（tGA）是一种不饱和的 C5-二羧酸，可能在 1 型戊二酸血症中积累，但其病理生理学仍不确定。在本工作中，研究了体外增加 tGA 浓度对大鼠大脑皮质神经化学和氧化应激参数的影响。我们观察到 tGA 降低了 Na(+)，K(+)-ATP 酶的活性，但对肌酸激酶、呼吸链复合物 IV 和 ATP 合酶的活性没有影响。另一方面，tGA 显著增加了脂质过氧化（硫代巴比妥酸反应物质水平和自发化学发光）和蛋白质氧化损伤（巯基氧化）。tGA 还显著降低了非酶抗氧化防御（TRAP 和还原型谷胱甘肽水平）。我们的数据表明，tGA 可能对大鼠大脑具有神经毒性。
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