[Influence of the length of cultivation of no early cleavage embryos on the IVF success rate].

OBJECTIVE OF THE STUDY

To evaluate the success of treatment cycle after transfer of no early cleavage embryos in relation to the length of in vitro cultivation.

TYPE OF THE STUDY

Retrospective study.

SETTING

Dept. of Obstetrics and Gynecology, Faculty of Medicine and Dentistry, Palacky University and University Hospital Olomouc.

METHODS

The IVF cycles with transfer of no early cleavage (NEC) embryos, ie. embryos with a delayed cleavage were evaluated in relation to the length of in vitro cultivation. The total of 338 embryos were cultured for three days (group 3D) and 204 embryos were cultured for 5 days (group 5D). The input parameters of the patients in these two groups were: their age, the percentage of fertilized oocytes and the number of transferred embryos. As the output parameters the number of cancelled cycles, clinical pregnancy rate (PR/ET), implantation rate (IR), and the number of pregnancy losses (AB) were evaluated. Finally, the results of these pregnancies were also evaluated.

RESULTS

Both study groups had the same input parameters, except that fewer embryos were transferred in the group 5D than in 3D (1.8 ± 0.5 vs. 2.0 ± 0.5,p = 0.000). No cycles were cancelled in the group 3D while 33 cycles were cancelled in 5D (p = 0.000). The number of clinical pregnancies and implantation rate in the group 5D was significantly higher than in 3D (PR/ET 50% vs. 37%, IR 36% vs. 25%, p = 0.001). The groups did not statistically significantly differ in the number of pregnancy losses, or in the number of pregnancies that resulted in childbirth.

CONCLUSION

In the 5D group with the longer in vitro cultivation statistically more pregnancies and implanted embryos were achieved, although the number of pregnancies that resulted in childbirth did not differ between groups.