弗氏链霉菌培养滤液对羽毛角蛋白的降解作用

Degradation of feather keratin by culture filtrates of Streptomyces fradiae.

作者信息

Young R A, Smith R E

出版信息

Can J Microbiol. 1975 May;21(5):583-6. doi: 10.1139/m75-084.

Abstract

Treatment of milled defatted capon feathers with filtrates of Steptomyces fradiae ATCC 10745 that had been concentrated 20 times showed that the most keratin degradation in 0.5h occurred at pH 8.0 and 60degrees C. If assay time was extended, however, adecrease in reaction rate occurred and appeared to result from keratinase instability at elevated temperatures. Exposure of commercial feather meal to unconcentrated culturefiltrates for 15 h produced greatest degradation at 50 degrees C.

摘要

用经20倍浓缩的弗氏链霉菌ATCC 10745的滤液处理研磨后的脱脂公鸡羽毛，结果表明，在0.5小时内，在pH值8.0和60摄氏度条件下，角蛋白降解最为显著。然而，如果延长测定时间，反应速率会降低，这似乎是由于高温下角蛋白酶不稳定所致。将商业羽毛粉暴露于未浓缩的培养滤液中15小时，在50摄氏度时产生的降解作用最大。