Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, 3000-548-Coimbra, Portugal.
Macromol Biosci. 2013 Jun;13(6):723-34. doi: 10.1002/mabi.201300017. Epub 2013 Apr 22.
Osteogenic/osteoinductive systems combine simvastatin, poloxamine Tetronic 908 (T908) and α-cyclodextrins (αCDs) in a supramolecular network that enhances the solubility/stability of the simvastatin hydroxy acid form and synergistically promotes osteoblast differentiation. Incorporation of 5% αCD transforms dilute T908 solutions (as low as 2% copolymer) into gels, enhances the osteoinductive activity of T908, and provides simvastatin sustained release for more than one week, which results in higher and more prolonged alkaline phosphatase (ALP) activity. The performance of the intrinsically osteoinductive polypseudorotaxane scaffold can be easily tuned by modifying the concentrations of T908, αCD, and simvastatin in a certain range of values. Moreover, the use of affordable, stable materials that can be sterilized applying a conventional method make the supramolecular gels advantageous candidates as scaffolds to be applied in the critical defect using minimally invasive techniques.
成骨/成骨诱导系统将辛伐他汀、聚氧乙烯四氢呋喃 908（T908）和α-环糊精（αCD）结合在一个超分子网络中，该网络提高了辛伐他汀羟酸形式的溶解度/稳定性，并协同促进成骨细胞分化。将 5%αCD 掺入可将稀释的 T908 溶液（低至 2%共聚物）转化为凝胶，增强 T908 的成骨诱导活性，并提供辛伐他汀的持续释放超过一周，从而导致更高和更持久的碱性磷酸酶（ALP）活性。通过在一定范围内改变 T908、αCD 和辛伐他汀的浓度，可以轻松调整固有成骨诱导的聚伪轮烷支架的性能。此外，使用价格合理、稳定的材料，并采用常规方法进行灭菌，使超分子凝胶成为具有优势的候选支架，可通过微创技术应用于临界缺陷。
