应用聚氧乙烯-环糊精超分子水凝胶修复骨临界缺损。

Bone critical defect repair with poloxamine-cyclodextrin supramolecular gels.

机构信息

Department of Chemical Engineering and Pharmaceutical Technology, University of La Laguna, 38200 La Laguna, Spain.

Department of Chemical Engineering and Pharmaceutical Technology, University of La Laguna, 38200 La Laguna, Spain; Institute of Biomedical Technologies (ITB), University of La Laguna, Spain.

出版信息

Int J Pharm. 2015 Nov 10;495(1):463-473. doi: 10.1016/j.ijpharm.2015.09.003. Epub 2015 Sep 8.

DOI:10.1016/j.ijpharm.2015.09.003

PMID:26362078

Abstract

The aim of this study was to evaluate the osteoinductive capacity of a poloxamine (Tetronic(®) 908, T) and α-cyclodextrin (αCD) supramolecular gel (T-CD) as scaffold in a critical size defect in rat calvaria. The T-CD gel was evaluated solely and after being loaded with simvastatin (SV) and bone morphogenetic protein (BMP-2) separately and in combinations in order to reduce the doses of the active substances. Three doses of SV (7.5, 75, 750 μg) and two doses of BMP-2 (3 and 6 μg) were tested. The histology and histomorphometrical analysis showed improved bone repair with T-CD compared to T, probably due to better release control of both SV and BMP-2. In addition, as T-CD eroded more slowly than poloxamine alone, it remained longer in the defect site. Although synergism was not obtained with BMP-2 and SV, according to the observed regeneration of the defect, the dose of BMP-2 and SV can be reduced to 3 μg and 7.5 μg, respectively.

摘要

本研究旨在评估聚氧乙烯醚（Tetronic®908，T）和α-环糊精（αCD）超分子凝胶（T-CD）作为支架在大鼠颅骨临界尺寸缺损中的成骨能力。T-CD 凝胶仅进行了评估，并在单独和分别加载辛伐他汀（SV）和骨形态发生蛋白（BMP-2）以及组合加载的情况下进行了评估，以减少活性物质的剂量。测试了三种剂量的 SV（7.5、75、750μg）和两种剂量的 BMP-2（3 和 6μg）。组织学和组织形态计量学分析表明，与 T 相比，T-CD 可改善骨修复，这可能是由于 SV 和 BMP-2 的释放控制更好。此外，由于 T-CD 比单独的聚氧乙烯醚侵蚀更慢，因此它在缺陷部位停留的时间更长。尽管 BMP-2 和 SV 没有协同作用，但根据观察到的缺陷再生，BMP-2 和 SV 的剂量可以分别减少至 3μg 和 7.5μg。