Department of BioNano Technology, Gachon University, Seongnam, Korea.
Int Neurourol J. 2013 Mar;17(1):2-10. doi: 10.5213/inj.2013.17.1.2. Epub 2013 Mar 31.
We present the construction of the lab-on-a-chip (LOC) system, a state-of-the-art technology that uses polymer materials (i.e., poly[dimethylsiloxane]) for the miniaturization of conventional laboratory apparatuses, and show the potential use of these microfluidic devices in clinical applications. In particular, we introduce the independent unit components of the LOC system and demonstrate how each component can be functionally integrated into one monolithic system for the realization of a LOC system. In specific, we demonstrate microscale polymerase chain reaction with the use of a single heater, a microscale sample injection device with a disposable plastic syringe and a strategy for device assembly under environmentally mild conditions assisted by surface modification techniques. In this way, we endeavor to construct a totally integrated, disposable microfluidic system operated by a single mode, the pressure, which can be applied on-site with enhanced device portability and disposability and with simple and rapid operation for medical and clinical diagnoses, potentially extending its application to urodynamic studies in molecular level.
我们介绍了芯片实验室（LOC）系统的构建，这是一种使用聚合物材料（例如聚二甲基硅氧烷）实现常规实验室仪器微型化的最先进技术，并展示了这些微流控设备在临床应用中的潜在用途。特别是，我们引入了 LOC 系统的独立单元组件，并展示了如何将每个组件功能集成到一个整体系统中，以实现 LOC 系统。具体来说，我们展示了使用单个加热器的微尺度聚合酶链反应、使用一次性塑料注射器的微尺度样品注入装置，以及在环境温和条件下通过表面改性技术辅助的设备组装策略。通过这种方式，我们努力构建一个完全集成的、一次性的微流控系统，由单一模式（压力）操作，可现场应用，具有增强的设备便携性和可处置性，以及简单快速的操作，用于医疗和临床诊断，可能将其应用扩展到分子水平的尿动力学研究。
