Postgraduate Institute of Medical Education and Research PGIMER, Department of Radiotherapy, Chandigarh 160012, India.
Biol Chem. 2013 Jul;394(7):889-900. doi: 10.1515/hsz-2012-0279.
Maackia amurensis agglutinin is a NeuNAcα (2-3) Galβ (1-4) GlcNAc/Glc-specific lectin, which was shown to have diagnostic potential in cancers of different origin. In a previous report, we demonstrated that GM3 specific IgG from bronchoalveolar lavage fluid (BALF) of non-small cell lung cancer (NSCLC) patients interacted with ∼66kDa membrane glycoprotein band of NSCLC cell lines, which was also recognised by this lectin. This observation prompted us to assess the potential of Maackia amurensis agglutinin in NSCLC. Accordingly, we examined the reactivity of this lectin with NSCLC cell lines as well as the tissue biopsies and cells obtained from fine needle aspirations of NSCLC patients. Maackia amurensis agglutinin showed strong reactivity, specifically with cells and biopsy samples of NSCLC origin. Furthermore, this lectin was found to induce apoptosis in NSCLC cells. The mechanism of this lectin-induced apoptosis involved downregulation of Bcl-XL, upregulation of Bax, release of cytochrome c and activation of procaspase-3. Collectively our results have suggested that Maackia amurensis agglutinin may have the potential to serve as a unique probe for detection of NSCLC and also as a specific apoptosis-inducing agent in NSCLC cells.
贺尔滨木霉菌凝集素是一种 NeuNAcα（2-3）Galβ（1-4）GlcNAc/Glc 特异性凝集素，已被证明在不同来源的癌症中有诊断潜力。在之前的一份报告中，我们证明了来自非小细胞肺癌（NSCLC）患者支气管肺泡灌洗液（BALF）的 GM3 特异性 IgG 与 NSCLC 细胞系的约 66kDa 膜糖蛋白带相互作用，该凝集素也能识别该带。这一观察结果促使我们评估贺尔滨木霉菌凝集素在 NSCLC 中的潜在应用。因此，我们检查了该凝集素与 NSCLC 细胞系以及来自 NSCLC 患者细针抽吸活检的组织和细胞的反应性。贺尔滨木霉菌凝集素表现出强烈的反应性，特别是与 NSCLC 起源的细胞和活检样本。此外，该凝集素被发现可诱导 NSCLC 细胞凋亡。该凝集素诱导凋亡的机制涉及 Bcl-XL 的下调、Bax 的上调、细胞色素 c 的释放和 procaspase-3 的激活。总的来说，我们的结果表明，贺尔滨木霉菌凝集素可能有潜力作为 NSCLC 检测的独特探针，也可能作为 NSCLC 细胞中特异性的凋亡诱导剂。
