Experimental Research Center, First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. China.
Oncol Rep. 2013 Jul;30(1):50-6. doi: 10.3892/or.2013.2417. Epub 2013 Apr 23.
As a novel member of the Ras family, ERas, found in murine embryonic stem (ES) cells in 2003, was considered a pseudogene. To date, there are a few reports on the relationship between ERas and tumors. It was recently suggested that ERas could affect gastric carcinoma (GC) metastasis, but no significant relationship was found with tumor proliferation. Since ERas plays an important role in tumor-like growth of ES cells subcutaneously injected into nude mice, we hypothesized that ERas plays a role in tumor proliferation. In this experiment, we selected 7 GC strains from different sources with different differentiation degrees, we detected the expression of full length ERas transcript, and selected two ERas highly expressing GC strains, MKN-28 and BGC-823. After knocking down the ERas gene by siRNA, we observed that there was a significant decrease in proliferation, metastasis as well as clonality. Therefore, ERas is confirmed to be an important gene in affecting tumor proliferation and metastasis. Furthermore, the significance of the ERas mechanism and signaling pathway is shown.
作为 Ras 家族的一个新成员，ERas 于 2003 年在鼠胚胎干细胞中被发现，被认为是一个假基因。迄今为止，关于 ERas 与肿瘤之间的关系仅有少数报道。最近有研究表明，ERas 可能影响胃癌（GC）的转移，但与肿瘤增殖没有明显关系。由于 ERas 在皮下注射到裸鼠的 ES 细胞的肿瘤样生长中发挥重要作用，我们假设 ERas 在肿瘤增殖中发挥作用。在本实验中，我们从不同来源、不同分化程度的 7 株 GC 株系中选择，检测全长 ERas 转录本的表达情况，并选择 2 株 ERas 高表达的 GC 株系 MKN-28 和 BGC-823。用 siRNA 敲低 ERas 基因后，我们观察到增殖、转移和克隆能力明显下降。因此，证实 ERas 是影响肿瘤增殖和转移的重要基因。此外，还展示了 ERas 机制和信号通路的意义。
