• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大鼠脑内多巴胺能和去甲肾上腺素能损伤后儿茶酚-O-甲基转移酶（COMT）蛋白表达和活性的变化。

Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain.

机构信息

Division of Pharmacology & Toxicology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.

出版信息

PLoS One. 2013 Apr 16;8(4):e61392. doi: 10.1371/journal.pone.0061392. Print 2013.

DOI:10.1371/journal.pone.0061392
PMID:23613844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3628796/
Abstract

The occurrence of catechol-O-methyltransferase (COMT) in presynaptic neurons remains controversial. This study utilized dopaminergic and noradrenergic toxins to assess the presence of COMT in the presynaptic neurons originating from the substantia nigra, ventral tegmental area or locus coeruleus. Destruction of dopaminergic and noradrenergic neurons was assessed by measuring the dopamine and noradrenaline content in the projection areas of these neurons. Additionally, COMT protein expression and activity were examined in several projection areas to determine whether there are any changes in COMT values. Colocalization studies were done to identify COMT-containing postsynaptic neurons. Despite successful lesioning of dopaminergic and noradrenergic neurons, no changes in COMT protein expression or activity could be noted. These results strongly suggest that COMT is not present in presynaptic dopaminergic and noradrenergic neurons. There was a high colocalization of COMT with the GABAergic marker of short neurons both in the striatum and cortex but only a weak, if any, with the cholinergic marker in the cortex.

摘要

儿茶酚-O-甲基转移酶（COMT）是否存在于突触前神经元中一直存在争议。本研究利用多巴胺能和去甲肾上腺素能毒素来评估源自黑质、腹侧被盖区或蓝斑的突触前神经元中 COMT 的存在。通过测量这些神经元投射区的多巴胺和去甲肾上腺素含量来评估多巴胺能和去甲肾上腺素能神经元的破坏情况。此外，还在几个投射区检查了 COMT 蛋白表达和活性，以确定 COMT 值是否有任何变化。进行共定位研究以鉴定含有 COMT 的突触后神经元。尽管多巴胺能和去甲肾上腺素能神经元的损伤成功，但未观察到 COMT 蛋白表达或活性的变化。这些结果强烈表明 COMT 不存在于突触前多巴胺能和去甲肾上腺素能神经元中。COMT 与纹状体和皮质中的短神经元的 GABA 标记物高度共定位，但与皮质中的胆碱能标记物的共定位仅为弱共定位（如果有）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/b2286ab9bd04/pone.0061392.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/70b7a9a41348/pone.0061392.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/8be749a02ca7/pone.0061392.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/cfb322a11aa8/pone.0061392.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/cb2cc4bac893/pone.0061392.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/3fdad5d7026a/pone.0061392.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/adfcd68d7e99/pone.0061392.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/b2286ab9bd04/pone.0061392.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/70b7a9a41348/pone.0061392.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/8be749a02ca7/pone.0061392.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/cfb322a11aa8/pone.0061392.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/cb2cc4bac893/pone.0061392.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/3fdad5d7026a/pone.0061392.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/adfcd68d7e99/pone.0061392.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117a/3628796/b2286ab9bd04/pone.0061392.g007.jpg

相似文献

1
Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain.大鼠脑内多巴胺能和去甲肾上腺素能损伤后儿茶酚-O-甲基转移酶（COMT）蛋白表达和活性的变化。
PLoS One. 2013 Apr 16;8(4):e61392. doi: 10.1371/journal.pone.0061392. Print 2013.
2
Potentiation of parkinsonian symptoms by depletion of locus coeruleus noradrenaline in 6-hydroxydopamine-induced partial degeneration of substantia nigra in rats.6-羟基多巴胺诱导大鼠黑质部分变性过程中，蓝斑去甲肾上腺素耗竭对帕金森症状的增强作用。
Eur J Neurosci. 2003 Jun;17(12):2586-92. doi: 10.1046/j.1460-9568.2003.02684.x.
3
Striatal membrane-bound and soluble catechol-O-methyl-transferase after selective neuronal lesions in the rat.大鼠选择性神经元损伤后纹状体膜结合型和可溶性儿茶酚-O-甲基转移酶
J Neural Transm. 1987;69(3-4):221-8. doi: 10.1007/BF01244343.
4
Catechol O-methyltransferase mRNA expression in human and rat brain: evidence for a role in cortical neuronal function.儿茶酚-O-甲基转移酶在人和大鼠大脑中的mRNA表达：其在皮质神经元功能中作用的证据
Neuroscience. 2003;116(1):127-37. doi: 10.1016/s0306-4522(02)00556-0.
5
Novel, non-nitrocatechol catechol-O-methyltransferase inhibitors modulate dopamine neurotransmission in the frontal cortex and improve cognitive flexibility.新型非硝基儿茶酚儿茶酚-O-甲基转移酶抑制剂调节前额叶皮质多巴胺能神经传递，改善认知灵活性。
Psychopharmacology (Berl). 2020 Sep;237(9):2695-2707. doi: 10.1007/s00213-020-05566-0. Epub 2020 May 30.
6
Subsecond Regulation of Synaptically Released Dopamine by COMT in the Olfactory Bulb.嗅球中儿茶酚-O-甲基转移酶对突触释放多巴胺的亚秒级调节
J Neurosci. 2016 Jul 20;36(29):7779-85. doi: 10.1523/JNEUROSCI.0658-16.2016.
7
Noradrenaline is crucial for the substantia nigra dopaminergic cell maintenance.去甲肾上腺素对于黑质多巴胺能细胞的维持至关重要。
Neurochem Int. 2019 Dec;131:104551. doi: 10.1016/j.neuint.2019.104551. Epub 2019 Sep 19.
8
Catecholamine metabolism in the brain by membrane-bound and soluble catechol-o-methyltransferase (COMT) estimated by enzyme kinetic values.通过酶动力学值估算的膜结合型和可溶性儿茶酚 - O - 甲基转移酶（COMT）对脑中儿茶酚胺代谢的影响。
Med Hypotheses. 2001 Nov;57(5):628-32. doi: 10.1054/mehy.2001.1430.
9
Neuronal expression of catechol O-methyltransferase mRNA in neonatal rat suprachiasmatic nucleus.新生大鼠视交叉上核中儿茶酚-O-甲基转移酶mRNA的神经元表达
Neuroreport. 2004 Jun 7;15(8):1239-43. doi: 10.1097/01.wnr.0000127635.38052.cd.
10
Immunohistochemical study of catechol-O-methyltransferase in the human mesostriatal system.人纹状体中间系统中儿茶酚-O-甲基转移酶的免疫组织化学研究。
Neuroscience. 1994 Sep;62(2):449-57. doi: 10.1016/0306-4522(94)90379-4.

引用本文的文献

1
Initial Molecular Mechanisms of the Pathogenesis of Parkinson's Disease in a Mouse Neurotoxic Model of the Earliest Preclinical Stage of This Disease.帕金森病最早临床前期阶段小鼠神经毒性模型中帕金森病发病的初始分子机制
Int J Mol Sci. 2024 Jan 22;25(2):1354. doi: 10.3390/ijms25021354.
2
Limiting Monoamines Degradation Increases L-DOPA Pro-Locomotor Action in Newborn Rats.限制单胺类降解可增加新生大鼠 L-DOPA 的促运动作用。
Int J Mol Sci. 2023 Sep 29;24(19):14747. doi: 10.3390/ijms241914747.
3
Should "on-demand" treatments for Parkinson's disease OFF episodes be used earlier?

本文引用的文献

1
Distribution of prolyl oligopeptidase in human peripheral tissues and in ovarian and colorectal tumors.脯氨酰寡肽酶在人外周组织及卵巢和结直肠肿瘤中的分布。
J Histochem Cytochem. 2012 Sep;60(9):706-15. doi: 10.1369/0022155412453051. Epub 2012 Jun 26.
2
Orientation and cellular distribution of membrane-bound catechol-O-methyltransferase in cortical neurons: implications for drug development.皮质神经元中膜结合儿茶酚-O-甲基转移酶的定位和细胞分布：对药物开发的影响。
J Biol Chem. 2011 Oct 7;286(40):34752-60. doi: 10.1074/jbc.M111.262790. Epub 2011 Aug 16.
3
The 6-hydroxydopamine-induced nigrostriatal neurodegeneration produces microglia-like NG2 glial cells in the rat substantia nigra.
帕金森病“关”期的“按需”治疗是否应更早使用？
Clin Park Relat Disord. 2022 Aug 12;7:100161. doi: 10.1016/j.prdoa.2022.100161. eCollection 2022.
4
Experimental Dopamine Reuptake Inhibitors in Parkinson's Disease: A Review of the Evidence.帕金森病中的实验性多巴胺再摄取抑制剂：证据综述
J Exp Pharmacol. 2021 Mar 29;13:397-408. doi: 10.2147/JEP.S267032. eCollection 2021.
5
Associations between catechol-O-methyltransferase (COMT) genotypes at rs4818 and rs4680 and gene expression in human dorsolateral prefrontal cortex.位于rs4818和rs4680的儿茶酚-O-甲基转移酶（COMT）基因多态性与人类背外侧前额叶皮质基因表达之间的关联。
Exp Brain Res. 2020 Feb;238(2):477-486. doi: 10.1007/s00221-020-05730-0. Epub 2020 Jan 20.
6
Quantitative Analysis of Catecholamines in the Pink1 -/- Rat Model of Early-onset Parkinson's Disease.在早发性帕金森病的 Pink1 -/- 大鼠模型中儿茶酚胺的定量分析。
Neuroscience. 2018 May 21;379:126-141. doi: 10.1016/j.neuroscience.2018.02.027. Epub 2018 Feb 27.
7
Metabolism of Dopamine in Nucleus Accumbens Astrocytes Is Preserved in Aged Mice Exposed to MPTP.伏隔核星形胶质细胞中多巴胺的代谢在接触MPTP的老年小鼠中得以保留。
Front Aging Neurosci. 2017 Dec 12;9:410. doi: 10.3389/fnagi.2017.00410. eCollection 2017.
8
Monoaminergic and Histaminergic Strategies and Treatments in Brain Diseases.脑部疾病中的单胺能和组胺能策略与治疗方法
Front Neurosci. 2016 Nov 24;10:541. doi: 10.3389/fnins.2016.00541. eCollection 2016.
6-羟多巴胺诱导的黑质纹状体神经退行性变使大鼠黑质中出现类似于小胶质细胞的 NG2 神经胶质细胞。
Glia. 2010 Nov 1;58(14):1686-700. doi: 10.1002/glia.21040.
4
Involvement of astroglial fibroblast growth factor-2 and microglia in the nigral 6-OHDA parkinsonism and a possible role of glucocorticoid hormone on the glial mediated local trophism and wound repair.星形胶质细胞源性成纤维细胞生长因子-2和小胶质细胞在黑质6-羟基多巴胺帕金森病中的作用以及糖皮质激素在胶质细胞介导的局部营养和伤口修复中的可能作用。
J Neural Transm Suppl. 2009(73):185-202. doi: 10.1007/978-3-211-92660-4_15.
5
Distribution of catechol-O-methyltransferase (COMT) proteins and enzymatic activities in wild-type and soluble COMT deficient mice.在野生型和可溶性儿茶酚-O-甲基转移酶（COMT）缺陷型小鼠中 COMT 蛋白和酶活性的分布。
J Neurochem. 2010 Jun;113(6):1632-43. doi: 10.1111/j.1471-4159.2010.06723.x. Epub 2010 Mar 31.
6
Importance of membrane-bound catechol-O-methyltransferase in L-DOPA metabolism: a pharmacokinetic study in two types of Comt gene modified mice.膜结合儿茶酚-O-甲基转移酶在 L-DOPA 代谢中的重要性：两种类型的 Comt 基因修饰小鼠的药代动力学研究。
Br J Pharmacol. 2009 Dec;158(8):1884-94. doi: 10.1111/j.1476-5381.2009.00494.x.
7
Distribution of cholinergic cells in guinea pig brainstem.豚鼠脑干中胆碱能细胞的分布。
Neuroscience. 2008 Jun 12;154(1):186-95. doi: 10.1016/j.neuroscience.2007.12.017. Epub 2008 Jan 28.
8
Increased catechol-O-methyltransferase activity and protein expression in OX-42-positive cells in the substantia nigra after lipopolysaccharide microinfusion.脂多糖微量注射后黑质中OX-42阳性细胞中儿茶酚-O-甲基转移酶活性和蛋白表达增加。
Neurochem Int. 2007 Nov-Dec;51(6-7):412-23. doi: 10.1016/j.neuint.2007.04.020. Epub 2007 May 6.
9
In heterozygous GDNF knockout mice the response of striatal dopaminergic system to acute morphine is altered.在杂合型胶质细胞源性神经营养因子基因敲除小鼠中，纹状体多巴胺能系统对急性吗啡的反应发生了改变。
Synapse. 2006 May;59(6):321-9. doi: 10.1002/syn.20245.
10
EVIDENCE FOR THE EXISTENCE OF MONOAMINE-CONTAINING NEURONS IN THE CENTRAL NERVOUS SYSTEM. I. DEMONSTRATION OF MONOAMINES IN THE CELL BODIES OF BRAIN STEM NEURONS.中枢神经系统中含单胺神经元存在的证据。I. 脑干神经元细胞体中单胺的证实。
Acta Physiol Scand Suppl. 1964:SUPPL 232:1-55.