Exp Dermatol. 2013 May;22(5):377-9. doi: 10.1111/exd.12147.
Keloid disease (KD) is a fibroproliferative disorder characterised partly by an altered extracellular matrix (ECM) profile. In fetal scarring, hyaluronic acid (HA) expression is increased, but is reduced in KD tissue compared with normal skin (NS). The expression of Hyaluronan Synthase (HAS) and hyaluronidase (HYAL) in KD and NS tissue were investigated for the first time using a range of techniques. Hyaluronan synthase and HYAL mRNA expression were significantly increased in NS tissue compared with KD tissue (P < 0.05). Immunohistological analysis of tissue indicated an accumulation of HAS and HYAL protein expression in KD compared with NS due to the thicker epidermis. No differences were observed in mRNA or protein expression in KD and NS fibroblasts. Reduced expression of HAS and HYAL may alter HA synthesis, degradation and accumulation in KD. Better understanding of the role of HA in KD may lead to novel therapeutic approaches to address the resulting ECM imbalance.
瘢痕疙瘩病 (KD) 是一种纤维增生性疾病，其特征部分在于细胞外基质 (ECM) 谱发生改变。在胎儿瘢痕中，透明质酸 (HA) 的表达增加，但与正常皮肤 (NS) 相比，KD 组织中的 HA 表达减少。首次使用一系列技术研究了 KD 和 NS 组织中透明质酸合酶 (HAS) 和透明质酸酶 (HYAL) 的表达。与 KD 组织相比，NS 组织中的透明质酸合酶和 HYAL mRNA 表达显著增加 (P < 0.05)。组织免疫组织化学分析表明，由于表皮较厚，KD 组织中 HAS 和 HYAL 蛋白表达的积累。KD 和 NS 成纤维细胞中未观察到 mRNA 或蛋白表达的差异。HAS 和 HYAL 的表达减少可能会改变 KD 中的 HA 合成、降解和积累。更好地了解 HA 在 KD 中的作用可能会导致针对 ECM 失衡的新的治疗方法。
