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利用葡萄球菌属分离菌对米糠进行阿魏酸的生物转化。

Biotransformation of rice bran to ferulic acid by pediococcal isolates.

机构信息

Punjabi University, Patiala, Punjab, India.

出版信息

Appl Biochem Biotechnol. 2013 Jun;170(4):854-67. doi: 10.1007/s12010-013-0223-1. Epub 2013 Apr 25.

Abstract

Ferulic acid (FA) is widely used in foods, in beverages, and in various pharmaceutical industries as a precursor of vanillin. FA biotransformation can occur during the growth of lactic acid bacteria (LAB), and its conversion to other phenolic derivatives is observed by many scientists, where ferulic acid esterase (FAE) and ferulic acid decarboxylase (FDC) play significant roles. The present study aimed at screening a panel of LAB for their ability to release FA from rice bran, an agro waste material. FAE and FDC activities were analyzed for the preliminary screening of various dairy isolates. Two Pediococcus acidilactici isolates were selected for studying further the hydrolysis of FA from rice bran and its bioconversion into phenolic derivatives like 4-ethylphenol, vanillin, vanillic acid, and vanillyl alcohol. P. acidilactici M16, a probiotic isolate, has great potential for the production of FA from rice bran and could be exploited as starter culture in the food industry for the production of biovanillin.

摘要

阿魏酸(FA)作为香草醛的前体,广泛应用于食品、饮料和各种制药工业中。在乳酸菌(LAB)的生长过程中会发生 FA 生物转化,许多科学家观察到其转化为其他酚类衍生物,其中阿魏酸酯酶(FAE)和阿魏酸脱羧酶(FDC)发挥着重要作用。本研究旨在筛选一组 LAB,以从农业废弃物米糠中释放 FA。对各种乳制品分离物进行了 FAE 和 FDC 活性分析,以进行初步筛选。选择了两种戊糖片球菌(Pediococcus acidilactici)分离株,进一步研究从米糠中水解 FA 及其生物转化为酚类衍生物,如 4-乙基苯酚、香草醛、香草酸和香草醇。益生菌分离株 P. acidilactici M16 具有从米糠中生产 FA 的巨大潜力,可作为食品工业中的起始培养物,用于生产生物香草醛。

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