Department of Biotechnology, Punjabi University, Patiala 147002, India.
Biomed Res Int. 2013;2013:590359. doi: 10.1155/2013/590359. Epub 2013 Aug 28.
Vanillin is widely used as food additive and as a masking agent in various pharmaceutical formulations. Ferulic acid is an important precursor of vanillin that is available in abundance in cell walls of cereals like wheat, corn, and rice. Phenolic biotransformations can occur during growth of lactic acid bacteria (LAB), and their production can be made feasible using specialized LAB strains that have been reported to produce ferulic acid esterases. The present study aimed at screening a panel of LAB isolates for their ability to release phenolics from agrowaste materials like rice bran and their biotransformation to industrially important compounds such as ferulic acid, 4-ethyl phenol, vanillic acid, vanillin, and vanillyl alcohol. Bacterial isolates were evaluated using ferulic acid esterase, ferulic acid decarboxylase, and vanillin dehydrogenase assays. This work highlights the importance of lactic acid bacteria in phenolic biotransformations for the development of food grade flavours and additives.
香兰素被广泛用作食品添加剂和各种药物制剂中的掩蔽剂。阿魏酸是香兰素的重要前体，在小麦、玉米和大米等谷物的细胞壁中含量丰富。在乳酸菌（LAB）生长过程中会发生酚类生物转化，使用已经报道能够产生阿魏酸酯酶的专用 LAB 菌株可以使其生产成为可行。本研究旨在筛选一组 LAB 分离株，以评估它们从稻糠等农业废料中释放酚类物质的能力，以及将其生物转化为工业上重要的化合物，如阿魏酸、4-乙基苯酚、香草酸、香草醛和香草醇的能力。使用阿魏酸酯酶、阿魏酸脱羧酶和香草醛脱氢酶测定法评估细菌分离株。这项工作强调了乳酸菌在酚类生物转化中的重要性，这对于开发食品级香料和添加剂具有重要意义。
