Glauser Gaëtan, Wolfender Jean-Luc
Chemical Analytical Service of the Swiss Plant Science Web, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
Methods Mol Biol. 2013;1011:123-34. doi: 10.1007/978-1-62703-414-2_10.
Upon wounding or herbivory, plants quickly react by activating various defense mechanisms. A major part of these defenses is thought to be regulated by the jasmonate pathway through the induction of jasmonic acid and its biologically active jasmonoyl-isoleucine conjugate. Yet, these well-known phytohormones are only two among the numerous compounds that compose the jasmonate family. Here, we describe a method based on ultrahigh-pressure liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry that can potentially profile the full range of known free and esterified jasmonates in a non-targeted manner. The developed approach is illustrated by the analysis of Arabidopsis thaliana leaves after mechanical wounding.
在受到创伤或植食性动物侵害时，植物会通过激活各种防御机制迅速做出反应。人们认为这些防御机制的主要部分是由茉莉酸途径调控的，该途径通过诱导茉莉酸及其具有生物活性的茉莉酰异亮氨酸共轭物来实现。然而，这些广为人知的植物激素只是构成茉莉酸家族的众多化合物中的两种。在此，我们描述了一种基于超高压液相色谱与四极杆-飞行时间质谱联用的方法，该方法有可能以非靶向方式对已知的游离和酯化茉莉酸类化合物进行全面分析。通过对机械损伤后的拟南芥叶片进行分析，展示了所开发的方法。
