泡沫病毒 Gag p71-p68 裂解是逆转录酶模板转换所必需的。
Foamy virus Gag p71-p68 cleavage is required for template switch of the reverse transcriptase.
机构信息
Universität Würzburg, Institut für Virologie und Immunbiologie, Würzburg, Germany.
出版信息
J Virol. 2013 Jul;87(13):7774-6. doi: 10.1128/JVI.00833-13. Epub 2013 Apr 24.
Abstract
In contrast to orthoretroviruses, processing of foamy viral p71 Gag is limited to a single cleavage site. Nevertheless, Gag maturation is essential for infectivity, but deletion of p3 results in a modest drop in infectivity. Here, we show that Gag processing of p71 to p68 and p3 is essential for full-length cDNA synthesis, while inactivation of Gag cleavage results in cDNAs containing only the RU5 region; cDNAs encompassing the U3 region were almost undetectable.
摘要
与 orthoretroviruses 相反，泡沫病毒 p71 Gag 的加工仅限于单一的切割位点。尽管如此，Gag 的成熟对于感染性是必不可少的，但是 p3 的缺失导致感染性略有下降。在这里，我们表明，p71 到 p68 和 p3 的 Gag 加工对于全长 cDNA 合成是必不可少的，而 Gag 切割的失活导致仅包含 RU5 区域的 cDNA；包含 U3 区域的 cDNA 几乎无法检测到。
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