Xiang Dan, Ai Weiming, Chen Xiao, Chen Yunyun, Chen Shaobo
Department of Marine Science, Wenzhou Medical College, Wenzhou 325035, PR China.
Mitochondrial DNA. 2013 Aug;24(4):356-8. doi: 10.3109/19401736.2012.760557. Epub 2013 Apr 25.
The complete mitogenome was first determined for a minnow Pseudohemiculter dispar in this study. It is 16,621 bp in length, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 control region. Overall nucleotide base composition is 30.30% A, 27.10% C, 17.10% G, and 25.50% T, with a A+T bias. Except for the tRNA-Ser2 that lost the dihydrouridine arm and replaced with one 11-bp loop, the other 22 tRNA genes can fold into the typical cloverleaf secondary structures. The origin of light-strand replication (OL) was found between the tRNA-Asn and tRNA-Cys genes, while the termination-associated sequence (TAS) was found in the control region. Both OL and TAS can form the hairpin structure that acts as the signals participated in the mitochondrial DNA replication.
本研究首次测定了麦穗鱼（Pseudohemiculter dispar）的完整线粒体基因组。其长度为16,621 bp，包括13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个控制区。总体核苷酸碱基组成为30.30%A、27.10%C、17.10%G和25.50%T，具有A+T偏好性。除tRNA-Ser2失去二氢尿嘧啶臂并被一个11 bp的环取代外，其他22个tRNA基因均可折叠成典型的三叶草二级结构。轻链复制起始点（OL）位于tRNA-Asn和tRNA-Cys基因之间，而终止相关序列（TAS）位于控制区。OL和TAS均可形成发夹结构，作为参与线粒体DNA复制的信号。
